Verified In-Situ Plate Seeding (VIPS) takes single cell cloning and image based clonality assurance to a whole new level of quality. This multi-tasking, compact instrument delivers a rock solid, ‘double lock’ solution for regulatory submission.
VIPS accelerates projects. High seeding efficiency for both 96 and 384-well plates, single round workflows for massively enhanced productivity and reduced timelines.
With VIPS you get healthier cells, more confirmed single cells per plate and better outgrowth. Two-round methodologies are a thing of the past.
Book a 30-minute Zoom session with a product expert
“For the VIPS, there’s so little media centered in the well that it makes it easy to locate the cell and prove the health authorities that the cell is clonal.”Thomas Kelly, Scientist at Janssen R&D LLC
“Demonstrating monoclonality has never been more reliable and straightforward. No more ambiguity, no more ghost cells. Both our clients and our own scientists are very pleased with the data the VIPS instrument generates.”Ferenc Boldog, Director of Cell Line Development
Operating at a pressure of less than one psi, VIPS gently dispenses cells in nanoliter droplets into a plate well. Crucially, the cell is imaged in the bottom of the dry well as evidence of seeding. Automated imaging in multiple tight z-stack layers of the whole droplet commences with intelligence-based image analysis confirming the existence of a single cell. The well is then filled automatically with media.
During the clonal growth phase, VIPS performs daily high-clarity whole-well imaging, recording a timeline of well images and performing analysis to measure confluency..Successful clonal outgrowth can be traced back to a single cell to confirm monoclonality.
For 96 and 384 well plates and quality single-project use consumables, we employ the simplest and safest method of single-cell dispensing, with no complex fluidics or mixed samples. VIPS uses low pressure to seed cells in nanoliter droplets into empty wells.
Using advanced image analysis technology, z-stack images are processed to confirm the presence of a single cell.
To assist in the verification of your clonality workflow, VIPS offers a verification methodology option based on rare-event fluorescence imaging.
A seeder and a separate imager?VIPS is a 2 in 1 system combining high efficiency seeding with high contrast whole well imaging, saving lab space and resources.
To complete the story, a clonality report is generated, cataloging both in data and images the whole story from single cell cloning to colony. The VIPS clonality report can be directly used as an important source of evidence, with a strong track record of IND submission success.
Manual limiting dilution methodologies are based on pipetting deliberately low cell/well concentrations to boost the probability of a single cell. With the advent of VIPS and image based assurance, credible evidence is gathered at multiple time points.
Designed specifically for the task, Solentim technology is honed for single cell imaging in the droplet by way of z-stack imaging and in the whole well. With high clarity images, even when near the cell wall, VIPS generates confidence for the lab and the regulator.
| Spec | Description |
| Dimensions (D x W x H) | 33.4 x 15.7 x 19 " |
| Net Weight | 83.77 lbs |
| Operating Conditions | Indoor use only Temperature: 50° F to 104° F Humidity: 20% to 80% non-condensing Altitude: Up to 2000M Mains Supply: +/- 10% rated voltage |
| Electrical Supply | Power Supply Input Voltage: 100-240V AV 50-60Hz Single Phase Power Supply Output Voltage: 24V DC – 250W Power Supply Input Connection: IEC Input |
VIPS Project Kits include the consumables parts needed for VIPS single cell seeding. Use these kits with VIPS to gently dispense cells in nanoliter droplets into empty wells for high efficiency single cell seeding. Each sterile kit includes a cell reservoir and a nozzle, media dispenser tubing and cell reservoir tubing. Kits are available for both the V1 cell reservoir nozzles and V2 cell reservoir nozzles. Please check which version has been set up with your instrument before selecting which kit to order.
• CC-2111 VIPS Project Kit with v1 Nozzle - One cell reservoir (including lid & stirrer), one v1 nozzle, one media dispenser tubing, one cell reservoir tubing.
• CC-2115 VIPS Project Kit with v2 Nozzle - One cell reservoir (including lid & stirrer), one v2 nozzle, one media dispenser tubing, one cell reservoir tubing.
In the search for high-value cells, growth conditions can be optimized to support early growth conditions. InstiGRO™ products are proprietary animal component-free cell culture supplements for CHO and HEK cells which confer major growth and survival advantages within cell line development. Designed to work with VIPS automation, Cell Metric® imaging or manual cell practices, InstiGRO can transform workflows with greatly enhanced successful colonies per plate.
View Product
High Viability iPSC Single Cell Cloning and Outgrowth
View Product
