MatriClone is a new matrix designed to support iPSC growth in single seeding workflows while maintaining pluripotency. MatriClone is optimized for ‘in-solution’ seeding and optically suited for automated imaging.
When used as part of the VIPS seeding protocol, MatriClone offers high levels of single cell survival and subsequent outgrowth, all in a simple, automated workflow which avoids the need for coating plates. MatriClone is a defined matrix consisting of a recombinant peptide and is animal component-free.
Why the need for change? Existing methodologies can include the likes of MatriGel, a chemically undefined mixture secreted from mouse sarcoma cells which must be manually coated onto plates.Often used with limiting dilution, a low efficiency statistical approach to single cell seeding, this approach is time-consuming and lacks in evidence of clonality.
MatriClone offers ideal optical quality to contribute to high-quality data when imaged by automated imaging platforms.This makes MatriClone ideally suited to workflows using post-seeding technologies including the Cell Metric® whole well imager.
Importantly, clones generated using the VIPS + MatriClone workflow have been shown by immunocytochemistry and karyotyping to maintain pluripotency after at least 10 passages and have retained their ability to successfully differentiate.
| RS-2100 | MatriClone – 2 x 175µg | |
| RS-2101 | MatriClone – 6 x 175µg | |
| RS-4100 | Clinical manufacturing grade laminin is now available for customers to use in combination with VIPS and Cell Metric instrumentation. | |
Single Cell Seeder
High efficiency, single cell seeding with image-based proof of clonality for GMP-compatible workflows
High contrast whole well imaging and documentation designed for single cell verification and clonal outgrowth monitoring.
