OsmoTECH® XT Single-Sample Osmometer | Advanced Instruments
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Looking for clinical solutions?

OsmoTECH® XT Single-Sample Micro-Osmometer

NOW AVAILABLE!

Best-in-class osmolality performance, designed with you in mind​

Increase your productivity and reliability with powerful freezing point depression technology for osmolality measurement with the all-new OsmoTECH XT single-sample osmometer.  The OsmoTECH XT measures the widest variety of sample types (high concentration, high viscosity) accurately across bioprocessing and cell & gene therapy workflows. It’s also designed to make your work as streamlined and comfortable as possible with even more robust data integrity and compliance features to match your digital environment. See how the OsmoTECH XT osmometer will change the way you work in the lab.

Highlighted features:
  • Offers the widest range of osmolality testing (0 – 4000 mOsm/kg H2O)
  • Large user-friendly interface​
  • New, intelligent freezing technology
  • Supports 21 CFR part 11, GMP and EU Annex 11 compliance
  • Meets Pharmacopeia osmolality testing guidelines

Overview

Industry Applications

biotech

IN BIOTECH LABS

Maximize biologic yield, quality, and purity by optimizing and ensuring the quality and consistency of your buffers, media, product, excipients, and process parameters.

food

IN FOOD & BEVERAGE LABS

Maintain efficiency while ensuring product quality and process consistency with osmolality testing from formulation to release.

PRODUCT FEATURES

OPTIMIZED LAB SPACE

Small footprint and integrated consumable shelf for consumables. Consumables are also clean-room friendly!

NEW, INTELLIGENT FREEZING TECHNOLOGY

With only 20 uL sample and an optimized cooling engine that is able to freeze to lower temperatures, measure even the most complex samples with even more accuracy and precision

ROBUST AND FLEXIBLE DATA MANAGEMENT

Several ways, including network share, USB download, Open Protocol Communication (OPC), LIS (TCP/IP) Communication, or optional printer

SUPPORTS COMPLIANCE

Data integrity features support 21 CFR part 11, GMP and EU Annex 11 compliance guidelines. Also meets Pharmacopeia osmolality testing guidelines.

EXPERIENCE WIDEST RANGE OF OSMOLALITY TESTING

Say goodbye to dilutions! With more than twice the osmolality range of its counterpart instrument, the OsmoTECH XT can measure the osmolality of highly concentrated solutions, incuding proteins and sugars.

LARGE USER-FRIENDLY INTERFACE

Like a smartphone, the interactive touch screen allows easy access to information.

MAINTAIN TRACEABILITY

The integrated barcode scanner helps with sample identification and reduces transcription errors.

ONE-STEP DIRECT SAMPLING

Positive displacement sampler helps aspirate even highly viscous sample types (hyaluronate, cryopreservatives, etc.).

Technical Specifications

Sample type Aqueous-based solution
Sample volume 20 ± 1 μL
Test time ≤150 seconds in low range; ≤190 seconds in high range
Sample Capacity Single sample
Resolution 1 mOsm/kg H2O
Range 0-4000 mOsm/kg H2O
Accuracy2 ±2 mOsm/kg H2O from nominal value between 0 and 400 mOsm/kg H2O (1 SD)
±0.5% from nominal value between 400 and less than 1500 mOsm/kg H2O (1 SD)
±1% from nominal value from 1500 to 4000 mOsm/kg H2O (1 SD)
Within-run repeatability3 Standard deviation ≤2 mOsm/kg H2O between 0 and 400 mOsm/kg H2O
Coefficient of variation ≤0.5% between 400 and less than 1500 mOsm/kg H2O
Coefficient of variation ≤1% from 1500 to 4000 mOsm/kg H2O
Supported Languages English, Simplified Chinese, French, German, Italian, Japanese, Korean, Russian, Spanish, Turkish
Storage temperature -20°C to +45°C (-4°F to +113°F)
Electrical voltage 100 to 240 VAC (50/60 Hz)
Power consumption 60 Watts
Dimensions (D x W x H) Not including test kit: 15″ x 14″ x 10″ (38 cm x 36 cm x 25 cm)

Including test kit: 15″ x 14″ x 11.5″ (38 cm x 36 cm x 38 cm)

Net weight 5.9 kg (12.9 lbs.)
Shipping weight 11.4 kg (25 lbs.)
Warranty One-year limited warranty on workmanship and parts
  1. Subject to change
  2. AI standards and reference solutions. Advanced Instruments completes
    testing from 0 mOsm/kg sample to 4000 mOsm/kg sample during
    in-house linearity testing.
  3. AI standards and reference solutions. Performance at Reference Conditions:
    20 °C to 25 °C (68 °F to 77 °F); 40 to 60% relative humidity

Associated Products

Osmometer Calibration Standards

Available from 0-4000 mOsm/kg H2O
10 x 2mL Ampules

Osmolality Linearity Set

100, 500, 900, 1500, 2000 mOsm/kg H2O
5x 2x 5mL Ampules

Osmometer Calibration Standards and Reference Solutions

SK-TECHXT OsmoTECH XT Convenience Kit (Includes: XT100, 3LA051, 3MA029, 3LA151, 3LA301, 3LA028, 3MA000, 3MA400)
3MA000 0 mOsm Calibration Standard, 10×2 mL Ampules View
3MA029 Clinitrol 290 Reference Solution, 10×2 mL Ampules View
3LA051 500 mOsm Calibration Standard, 10×5 mL Ampules View
3LA151 1500 mOsm Calibration Standard, 10×5 mL Ampules View
3LA301 3000 mOsm Calibration Standard, 10×5 mL Ampules View
3MA400 4000 mOsm Calibration Standard, 10×2 mL Ampules View
3LA028 Osmolality Linearity Set, 100-2000 mOsm 5x2x5 mL Ampules View

Supplies

XT100 Micro-Sample Test Kit: 1 box of 100 chamber cleaners, 100 sampler tips, 1 plunger wire
3M0828A Plunger Wire, 1 per order (Replaces 3M0828 – Plunger Wires, set of 2. Please note: A plunger wire is included in every 133800 and XT100 Micro-Sample Test Kit box)
3M0825 Ease-eject 20-Microliter Sampler
240821 Sampler Tips, 50/pkg

Accessories

135022 Epson Dot Matrix Printer (sold-separately)
AN2TP5 Dot Matrix Printer Paper

Biotech Services

Get the best performance from your osmometer from the people who designed and built it. We provide a range of services and service plans to meet the different needs of our customers. Our certified Field Service Engineers provide on-site professional support for the installation, training, validation, and maintenance of your osmometers.

Applications

When it comes to process development and manufacturing of protein therapeutics, whether you’re making an antibody-based or other protein therapeutic, what you don’t know can slow you down.

  • Are all of your buffers and media formulated correctly?
  • Does your solution have unexpected components?
  • Are all of the steps involving solutions–i.e. cell growth, dialysis, chromatography–proceeding consistently and as expected?

With the innovative osmometers from Advanced Instruments, you can gain valuable data for managing process and product quality through a simple 90-second test. Osmolality, the measurement of total solute concentration, is a reliable control specification and in-process parameter that can deliver information across the protein therapeutic process development and manufacturing workflow.

See all Advanced Osmometers >

Upstream

Optimize cell culture conditions to maximize cell density and yield.

Cell-Line-Development_TItle

Cell Line Development

Ensure that culture conditions are compatible with your clone and cell culture system contributing to optimal cell density and tite.
Media-Preparation_Title

Media Preparation

Check media composition for optimal cell growth.
Cell-CultureMonitoring_Title

Cell Culture Monitoring

Ensure that culture conditions are correct for proper growth and health of cells. This promotes maximum density and yield.
Downstream

Monitor buffer composition and processes, such as chromatography and filtration, to ensure protein purity and yield.

BufferPreparation_Title

Buffer Preparation

Check for proper buffer composition for efficient processing and optimal quality and yield.
Chromatography_Title

Chromatography

Maintain the purity of the drug intermediates and identify and process deviations that could compromise product yield.
Buffer-Exchange_TItle

Buffer Exchange

Monitor buffer composition and verify proper exchange to optimize the number of diavolumes and acquire the highest product yield with the highest quality.
Fill & Finish

Monitor and verify the concentration of API and excipients to meet internal quality and regulatory requirements

FormulationDevelopment_title

Formulation Development

Optimize formulation concentration and stability for high yield in manufacturing.
Final-Formulation_Title

Final Formulation

Monitor and/or verify the concentration of excipients. Confirm soluble content of formulations. Minimize injection site pain.
Filtration_and_Fill_Title

Filtration and Fill

Key compendial release specification to confirm product stability.

When the therapeutic you’re developing is cell-based, constant monitoring to optimize growth and ensure consistent culture conditions throughout multiple stages is critical for success.

  • Are your stem cells at highest viability and product quality levels throughout culture and passaging?
  • Are you ensuring a high rate of cell recovery post-thaw?
  • Are your transduction mechanics being optimized?

Using osmolality can help your group answer these questions early in the process, ultimately helping you save time, money, and limited or costly reagents. Adding a simple 90-second osmolality measurement to the in-process parameters you measure is a quick and easy way to ensure optimal cell growth, during process development and manufacturing as well as during buffer and media preparation.

With the innovative osmometers from Advanced Instruments, you can keep delicate cells at optimal growth and gain valuable data for managing process and product quality across the cell therapy process development and manufacturing workflow.

See all Advanced Osmometers >

Upstream

Characterize media and optimize transduction of cells with lentivirus to optimize cell viability and size.

Cell-Line-Development_TItle

Cell Line Development

Ensure appropriate trending by monitoring small-scale cell culture for some cell therapies.
Media-Preparation_Title

Media Preparation

Characterize media formulation and QC raw materials for optimal cell growth.
Cell-CultureMonitoring_Title

Cell Culture Monitoring

Ensure that culture conditions are correct for proper growth and health of cells. This promotes maximum cell viability.
Downstream

Optimize downstream processing by confirming buffer concentrations and minimizing shock to cells.

BufferPreparation_Title

Buffer Preparation

Check for proper buffer composition for efficient processing and optimal quality and yield.
Chromatography_Title

Chromatography

Maintain purification conditions to maximize yield and protect cells.
Buffer-Exchange_TItle

Buffer Exchange

Optimize solution concentrations for UF/DF concentration and buffer exchange to acquire the highest product yield with the highest quality.
Fill & Finish

Monitor and ensure final composition and manage cryopreservation and storage.

FormulationDevelopment_title

Formulation Development

Optimize formulation concentration and composition drug safety.
Final-Formulation_Title

Final Formulation

Confirm concentration of formulations and ensure compatibility of cells with cryopreservatives.
Filtration_and_Fill_Title

Formulation and Fill

Key compendial release specification to confirm stability.

Consistency is critical when producing genetic therapies, including viral vector delivery and gene editing.  Small deviations in the composition of cell growth media can have unwanted effects on cell density, cell composition, and virus yield.

  • Are you looking for ways to increase vector stability?
  • Is your gene therapy group looking for higher transfection efficiency and higher viral vector production?

Using osmolality can help your group work towards those goals, as well as answer:

    • how to optimize the physiological range for transfection complexes
    • identifying media needs
    • determining effects of osmotic pressure on the stability of a complex

With the innovative osmometers from Advanced Instruments, you can gain valuable data for managing process and product quality through a simple 90-second test. When done right, osmolality, the measurement of total solute concentration, is a reliable control specification and in-process parameter that can deliver information across the gene therapy process development and manufacturing workflow.

See all Advanced Osmometers >

Upstream

Optimize cell culture conditions and media selection to maximize cell density, size and, ultimately, virus yield.

Cell-Line-Development_TItle

Cell Line Development

Ensure that culture conditions are compatible with your clone and cell culture system, contributing to optimal cell density and titer. Maximize transfection efficiency for optimal yield.
Media-Preparation_Title

Media Preparation

Characterize media preparation and composition for optimal cell growth.
Cell-CultureMonitoring_Title

Cell Culture Monitoring

Ensure that culture conditions are correct for proper growth and health of cells. This promotes maximum cell density and yield.
Downstream

Check raw material concentration and perform in-process monitoring of purification and filtration steps to maximize purity, cell/virus stability, and yield.

BufferPreparation_Title

Buffer Preparation

Check for proper buffer composition for efficient processing and optimal quality and yield.
Chromatography_Title

Chromatography

Maintain the purity of the drug intermediates, protect the viral vector and genetic content within the vector during process, and identify process deviations that could compromise product yield.
Buffer-Exchange_TItle

Buffer Exchange

Monitor buffer composition and verify proper exchange to optimize the number of diavolumes and acquire the highest product yield with the highest quality.
Fill & Finish

Monitor and ensure final composition, dosage, and stability

FormulationDevelopment_title

Formulation Development

Optimize formulation concentration and composition for high yield in manufacturing.
Final-Formulation_Title

Final Formulation

Monitor and/or verify the concentration of excipients. Confirm soluble content (solute concentrations) of formulations. Ensure compatibility of precious product with cryopreservatives.
Filtration_and_Fill_Title

Filtration and Fill

Key compendial release specification to confirm product dosage specifications.

Osmolality testing has unlimited applications in areas of personal care and hygiene. Products that come into contact with or penetrate the skin must have an osmolality within a physiological range to avoid damaging cells and tissues. In some instances, slightly hypo- or hyper-osmotic solutions are generated to promote wetting or drying effects. The manufacturing of skincare products, personal lubricants and optical products

One key to a youthful look is healthy and moisturized skin. Proper hydration of skin depends on a balance of water flow into and out of skin cells and, thus, depends on osmolality. Osmolality testing of skincare products provides assurance that the product will have moisturizing effects and serves as a control during the production process.

The osmolality of personal lubricant formulations impacts their effect and safety. The World Health Organization (WHO) stated that the osmolality of these solutions should not exceed 1200 mOsm/kg, with this limit decreasing to a more iso-osmotic level in the future.

Similar value for osmolality testing can be clearly seen in optical applications. Eye drops and other optical products are designed to lubricate or protect the eye. The goal of these solutions (e.g. adding moisture, removing moisture, etc.) dictates an ideal osmolality range.

See all Advanced Osmometers >

References

  • Aragona P et al. Physiochemical Properties of Hyaluronic Acid-Based Lubricant Eye Drops. Trans Vis Sci Tech. 2019;8(6):2.
  • Corrales R et al. Effects of Osmoprotectants on Hyperosmolar Stress in Cultured Human Corneal Epithelial Cells. Cornea 2008;27:574-579.
  • WHO/UNFPA/FHI, 2011-. Advisory Committee Notes to the Female Condom Technical Review Committee Meeting. [Geneva, Switzerland].

Osmolality testing is required to ensure that the final vaccine product is safe for administration. The osmolality must be within a physiological range to be introduced in human tissues. There is also evidence that injectable formulations with high osmolality may cause pain at the site of injection.

Vaccines are derived from a variety of sources, both living and synthetic, meaning a variety of bioproduction workflows are required. These workflows include some common use cases for osmolality testing, including the following:

  • Upstream cell culture and/or cell expansion requires maintaining an environment at the appropriate osmolality for the specific cell type. For those modalities that are cell-based, regular osmolality testing will ensure that the media and environment are optimized.
  • Buffers used during any downstream processing should be tested for osmolality to verify the concentrations of the solutions that are meant to protect the drug substance throughout the chromatography and/or filtration operations.
  • Final product testing must include osmolality to verify identify and safety of any injectable.

Differentiation in use cases for osmolality testing are outlined below:

Monoclonal Antibody Vaccines

Osmolality testing should be performed on hybridoma cell media to ensure optimal antibody production rates.

  • The rate of antibody production in hybridoma cells depends on the osmolality of the medium in which the cells are grown.
  • The osmotic agent used to affect the osmolality levels also impacts Ab production.

Viral Vaccines

Osmolality testing should be performed on media solutions to optimize cell size and viral vector stability.

  • Osmotic pressure affects the size of the morphology of insect cells and viruses.
  • The osmolality of the media affects the composition of the lipid bilayer membrane of enveloped viruses. This directly impacts the vector stability.

mRNA and DNA Vaccines

Delivery systems and mechanisms should be tested for osmolality.

  • mRNA can be complexed with different agents (e.g. PEI) for delivery within the body. Osmolality impacts the stability of the complex of viral models.
  • Osmolality impacts the ability to penetrate the target cell membrane.

COVID-19 vaccine development often falls into these development categories. To better understand how osmolality testing benefits the production of therapies, visit the respective tabs: Protein Therapies, Cell Therapies, Gene Therapies, Oligonucleotides.

See all Advanced Osmometers >

An example of an accelerated mRNA vaccine production workflow is below:

Accelerated Development
(as little as 1 year)

References

This novel field involves the use of antisense oligonucleotides (ASOs), aptamers, siRNAs, miRNAs and synthetic mRNAs to control the expression of genes that have been associated with diseases. Multiple Advanced Instruments customers within this field use osmometers to verify the concentration of the solutions carrying their precious products. Ultimately, the quality of the drug is dependent on the process controls implemented throughout the workflow. Below are some of the current uses for osmolality testing in this field:

Buffer QC

Osmolality specifications for buffers to preserve and protect the critical quality attributes of the product as it is being developed.

Final Product

Compendial release test needed to confirm the identity and concentration of the oligo product for safe use.

Because this is an emerging field, it comes with a number of development and application challenges:

  • Delivery of RNAs across the lipid bilayer and into cells
  • Immunogenicity once in the body
  • High rates of degradation of oligos by plasma and tissues

Drug delivery into the target cells requires the proper osmotic pressure, similar to the parameters around viral transfection in vector development. The optimal osmolality of the delivery complex (e.g. mRNA:PEI) should be determined and monitored during this process. This specification will provide repeatable successful delivery of the drug.

See all Advanced Osmometers >

References

Comparison Guide

All models support

Pharmacopeia testing guidelines

Being discontinued Dec. 31, 2020
Workflow Features
Best for labs with daily sample processing needs of: >10/day >10/day 1-10/day 1-10/day 1-10/day
Sample volume 100 µL 30 µL 20 µL 20 µL 250 µL
Sample introduction Automated liquid handling sample cup (automated) Sample cup One-step direct One-step direct Sample cup
Operating range (mOsm/kg H2O) 0 – 4,000 0 – 2,000 0 – 4,000 0 – 2,000 0 – 4,000
Test time (seconds) 180 90 Less than 150 in low range, Less than 180 in high range 90 120
Compliance Support Features
Supports 21 CFR Part 11 & EU Annex 11 compliance
Supports Pharmacopeia testing guidelines compliance
Instrument Features
Integrated barcode scanner
Printer
Multi-language touchscreen display
User name & password compatibility
Multi-sample capacity
Automated cleaning between samples
Automated sample pipetting
Temperature control method Dry cooling (No heat transfer fluid) Dry cooling (No heat transfer fluid) Dry cooling (No heat transfer fluid) Dry cooling (No heat transfer fluid) Heat-transfer fluid
Data Management Features
TCP/IP
USB
Webserver
OPC-UA, Network share,
Database backup
DTE RS-232, Barcode port
Part # A2O OsmoTECHPRO OsmoTECHXT OsmoTECH 3250
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Educational Resources

Brochure

OsmoTECH® XT Single-Sample Micro-Osmometer

Q&A Resource

Data Integrity & Compliance – what every lab needs to know

Scientific Resource

8 Ways that Osmolality Testing Improves Cell & Gene Therapy Processing

We are open and continue to provide essential lab supplies during the COVID-19 pandemic. Answers to your FAQ’s can be found here.

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