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A₂O® Advanced Automated Osmometer

Built to keep the busiest labs productive, the A2O Advanced Automated Osmometer combines exceptional analytical performance with full automation. Laboratories can depend on the A2O to deliver accurate and precise osmolality measurements while maximizing walk-away time.

Highlighted features:
  • Full automation
  • Gold standard freezing point depression technology
  • Supports CAP & CLIA compliance

Overview

Industry Applications

clinical

IN CLINICAL CHEMISTRY LABS

The premier osmometer for reliable high-throughput osmolality testing of patient samples to drive efficiency for your lab. The A2O delivers the results security, connectivity, quality control and ease of use to maximize productivity and keep the busiest clinical chemistry labs in compliance.

biotech

IN BIOTECH LABS

The full automation of the A2O keeps your bioprocess development and biologics manufacturing workflows on track with minimum hands-on time. Its exceptional analytical performance helps ensure the highest levels of quality, purity and yield for your biologic product.

food

IN FOOD AND BEVERAGE LABS

Maximize resources and ensure consistent product quality and process consistency with osmolality testing from formulation to release.

PRODUCT FEATURES

AUTOMATED MULTI-SAMPLE CAPABILITY

An intelligently designed 20-position sample tube carousel accommodates the most common laboratory sample tube sizes and shapes. Low volume inserts and nesting cups can also be used.

TOUCH-SCREEN USER INTERFACE

With a menu-driven operating system, intuitive software control, and multi-language capability, the A2O provides a simple operating experience at your fingertips.

DIRECT PRIMARY TUBE SAMPLING

The instrument automatically transfers 100 µL into the analyzer, eliminating the manual pipetting step for improved accuracy and reduced sampling error.

INTELLIGENT LIQUID HANDLING TECHNOLOGY

The pipette system features liquid level sensing and crash detection circuitry, eliminating the guesswork involved with sample preparation.

AUTOMATED SELF-CLEANING

The closed-loop fluid management system in the A2O cleans after each sample to eliminate sample carryover and cross contamination.

POSITIVE SAMPLE IDENTIFICATION

Use the integrated bar code scanner to eliminate transcription errors or enter sample IDs manually. The onboard printer provides additional sample identification and results reporting benefits.

FLEXIBLE SAMPLING CAPABILITIES

Program the A2O to process samples in duplicate, triplicate, or any sampling protocol your laboratory requires.

PROTECT YOUR DATA

Active Directory/LDAP options minimize manual input and the risk of errors. Automatic or manual backups, three levels of user access, and an automatic logout timer keep your data safe during idling. Plus, you get unlimited results and events storage

EASILY INTEGRATE DATA MANAGEMENT INTO YOUR ESTABLISHED WORKFLOW

Quickly save data to a network database, USB device, or the embedded Web server and eliminate the need to manually manage date and time by synchronizing with network time.

ENHANCED LIS AND DATA MANAGEMENT CAPABILITY

The A2O features bi-directional data communications as well as an on-board results database with search capability. Connectivity is a snap with Ethernet and multiple USB ports.

BUILT IN QUALITY CONTROL

The A2O comes complete with a full list of QC features including: A supervisor mode with password protection, ability to set range limits for control solutions, statistical monitoring of daily QC, and a system events log for diagnostic purposes.

BACKED BY COMPREHENSIVE SUPPORT

You can purchase any Advanced Instruments osmometer with complete confidence as every instrument is backed by a knowledgeable, experienced, and responsive customer support team.

Technical Specifications

Sample test volume100μL
Aspirated Sample volume150μL
Sample capacity20 sample carousel
Test timeLess than 3 minutes
Sample throughputcan process 20 samples in less than 1 hour
UnitmOsm/kg H2O
Calibrated Rangelow: 0 to 2000 mOsm/kg H2O
full: 0 to 4000 mOsm/kg H2O
Resolution1 mOsm/kg H2O
Calibrated3-point Calibrated for low range
4-point for full range
communicationOnbord printer, 10/100Mbps Ethernet, 4 USB 1.0/1.1/2.0 integrated bar code scanner
Accuracy0 to 400 mOsm/kg H2O: mean value ≤ 2 mOsm/kg H2O from nominal value 400-4000 mOsm: mean value ≤ 0.5% from normal value
precision (Within-run)standard deviation ≤ 2 mOsm/kg H2O between 0 and 400 mOsm; standard deviation ≤ 0.5% of value between 400 to 4000 mOsm
DriftLess than 1 mOsm/kg H2O per month
Temperature effects1Recalibration recommended for ambient temperature change 5°C (9°F) or greater
Storage temperature-40°C to +45°C (-40°F to +113°F)
Simple ViscosityUp to 20 mPa-s(20 cP) higher viscosities may affect pipettor accuracy
Pipettor Accuracy< 1%
Electrical voltage100-240 VAC (50/60 Hz)
Power consumption375W
Dimensions (D x W x H)20.5″ x 23.6″ x 22.8″ (52 cm x 60 cm x 58 cm)
Net weight68 lb (31 kg)
Shipping weight133 lb (60 kg)
WarrantyOne-year limited warranty on workmanship and all parts except glass, plastic, and part warrantied by their makers
*Operating condition -18°C to 35°C (64°F to 95°F); 5% to 80% relative humidity (noncondensing)
*specifications subject to change

Associated Products

Osmometer Calibration Standards

100, 900, 2000, 3000 mOsm
10 x 5mL Ampules

Osmolality Linearity Set

100-2,000 mOsm
5x 2x 5mL Ampules

Supplies & Accessories

200005UGUser’s Guide
200220Disposable 12×75 mm Sample Test Tubes, Box 250
200221Probe Wiper Discs, Box 50
200222Osmometer System Fluid, 1 Bottle 500 mL
200223Disposable Sample Tubes, Box 500
FLA835Thermal Printer Paper, 5 rolls

Osmometer Calibration Standards & Reference Solutions

3LA011100 mOsm Calibration Standard, 10×5 mL AmpulesView
3LA091900 mOsm Calibration Standard, 10×5 mL AmpulesView
3LA2012000 mOsm Calibration Standard, 10×5 mL AmpulesView
3LA3013000 mOsm Calibration Standard, 10×5 mL AmpulesView
3MA029Clinitrol 290 Reference Solution, 10×2 mL AmpulesView
3LA028Osmolality Linearity Set 100-2,000 mOsm 5x2x5 mLView

Osmometer Control Solutions

3MA028Protinol 3-Level Osmometer Control, 3x3x3 mLView
3LA085Renol 2-Level Osmometer Control, 2x4x3 mLView

Services

Get the best performance from your osmometer from the people who designed and built it. We provide a range of services and service plans to meet the different needs of our customers. Our certified Field Service Engineers provide on-site professional support for the installation, training, validation, and maintenance of your osmometers.

Applications

Resources for applications of using osmolality within biotechnology processes and manufacturing, clinical chemistry and product quality assurance within food & beverage.

When it comes to process development and manufacturing of protein therapeutics, whether you’re making an antibody-based or other protein therapeutic, what you don’t know can slow you down.

  • Are all of your buffers and media formulated correctly?
  • Does your solution have unexpected components?
  • Are all of the steps involving solutions–i.e. cell growth, dialysis, chromatography–proceeding consistently and as expected?

Osmolality, the measurement of total solute concentration, is a reliable control specification and in-process parameter that can deliver information across the protein therapeutic process development and manufacturing workflow.

Upstream

Optimize cell culture conditions to maximize cell density and yield.

Cell-Line-Development_TItle

Cell Line Development

Ensure that culture conditions are compatible with your clone and cell culture system contributing to optimal cell density and tite.
Media-Preparation_Title

Media Preparation

Check media composition for optimal cell growth.
Cell-CultureMonitoring_Title

Cell Culture Monitoring

Ensure that culture conditions are correct for proper growth and health of cells. This promotes maximum density and yield.
Downstream

Monitor buffer composition and processes, such as chromatography and filtration, to ensure protein purity and yield.

BufferPreparation_Title

Buffer Preparation

Check for proper buffer composition for efficient processing and optimal quality and yield.
Chromatography_Title

Chromatography

Maintain the purity of the drug intermediates and identify and process deviations that could compromise product yield.
Buffer-Exchange_TItle

Buffer Exchange

Monitor buffer composition and verify proper exchange to optimize the number of diavolumes and acquire the highest product yield with the highest quality.
Fill & Finish

Monitor and verify the concentration of API and excipients to meet internal quality and regulatory requirements

FormulationDevelopment_title

Formulation Development

Optimize formulation concentration and stability for high yield in manufacturing.
Final-Formulation_Title

Final Formulation

Monitor and/or verify the concentration of excipients. Confirm soluble content of formulations. Minimize injection site pain.
Filtration_and_Fill_Title

Filtration and Fill

Key compendial release specification to confirm product stability.

References

Consistency is critical when producing genetic therapies, including viral vector delivery and gene editing.  Small deviations in the composition of cell growth media can have unwanted effects on cell density, cell composition, and virus yield.

  • Are you looking for ways to increase vector stability?
  • Is your gene therapy group looking for higher transfection efficiency and higher viral vector production?

Using osmolality can help your group work towards those goals, as well as answer:

    • how to optimize the physiological range for transfection complexes
    • identifying media needs
    • determining effects of osmotic pressure on the stability of a complex
Upstream

Optimize cell culture conditions and media selection to maximize cell density, size and, ultimately, virus yield.

Cell-Line-Development_TItle

Cell Line Development

Ensure that culture conditions are compatible with your clone and cell culture system, contributing to optimal cell density and titer. Maximize transfection efficiency for optimal yield.
Media-Preparation_Title

Media Preparation

Characterize media preparation and composition for optimal cell growth
Cell-CultureMonitoring_Title

Cell Culture Monitoring

Ensure that culture conditions are correct for proper growth and health of cells. This promotes maximum cell density and yield.
Downstream

Check raw material concentration and perform in-process monitoring of purification and filtration steps to maximize purity, cell/virus stability, and yield.

BufferPreparation_Title

Buffer Preparation

Check for proper buffer composition for efficient processing and optimal quality and yield.
Chromatography_Title

Chromatography

Maintain the purity of the drug intermediates, protect the viral vector and genetic content within the vector during process, and identify process deviations that could compromise product yield.
Buffer-Exchange_TItle

Buffer Exchange

Monitor buffer composition and verify proper exchange to optimize the number of diavolumes and acquire the highest product yield with the highest quality.
Fill & Finish

Monitor and ensure final composition, dosage, and stability

FormulationDevelopment_title

Formulation Development

Optimize formulation concentration and composition for high yield in manufacturing.
Final-Formulation_Title

Final Formulation

Monitor and/or verify the concentration of excipients. Confirm soluble content (solute concentrations) of formulations. Ensure compatibility of precious product with cryopreservatives.
Filtration_and_Fill_Title

Filtration and Fill

Key compendial release specification to confirm product dosage specifications.

When the therapeutic you’re developing is cell-based, constant monitoring to optimize growth and ensure consistent culture conditions throughout multiple stages is critical for success.

  • Are your stem cells at highest viability and product quality levels throughout culture and passaging?
  • Are you ensuring a high rate of cell recovery post-thaw?
  • Are your transduction mechanics being optimized?

Using osmolality can help your group answer these questions early in the process, ultimately helping you save time, money, and limited or costly reagents. Adding a simple 90-second osmolality measurement to the in-process parameters you measure is a quick and easy way to ensure optimal cell growth, during process development and manufacturing as well as during buffer and media preparation.

Upstream

Characterize media and optimize transduction of cells with lentivirus to optimize cell viability and size.

Cell-Line-Development_TItle

Cell Line Development

Ensure appropriate trending by monitoring small-scale cell culture for some cell therapies.
Media-Preparation_Title

Media Preparation

Characterize media formulation and QC raw materials for optimal cell growth.
Cell-CultureMonitoring_Title

Cell Culture Monitoring

Ensure that culture conditions are correct for proper growth and health of cells. This promotes maximum cell viability.
Downstream

Optimize downstream processing by confirming buffer concentrations and minimizing shock to cells.

BufferPreparation_Title

Buffer Preparation

Check for proper buffer composition for efficient processing and optimal quality and yield.
Chromatography_Title

Chromatography

Maintain purification conditions to maximize yield and protect cells.
Buffer-Exchange_TItle

Buffer Exchange

Optimize solution concentrations for UF/DF concentration and buffer exchange to acquire the highest product yield with the highest quality.
Fill & Finish

Monitor and ensure final composition and manage cryopreservation and storage.

FormulationDevelopment_title

Formulation Development

Optimize formulation concentration and composition drug safety.
Final-Formulation_Title

Final Formulation

Confirm concentration of formulations and ensure compatibility of cells with cryopreservatives.
Filtration_and_Fill_Title

Formulation and Fill

Key compendial release specification to confirm stability.

For laboratories seeking premium, reliable osmometers that meet their everyday needs for workflow efficiency, result security and integrity, connectivity, quality control, and audit control, Advanced Osmometers are the right choice.

Keep your labs running smoothly and in compliance with osmometers that use the industry preferred freezing point depression method to provide accurate patient results.

Upcoming Trade Shows

  1. CLMA KnowledgeLab | 29-31 March 2020 | Louisville, KY
  2. AACC 72nd Annual Scientific Meeting | 26-30 July 2020 | Chicago, IL

Ensure optimal product consistency with quick and accurate osmolality measurement using an A2O Advanced Automated Osmometer. Designed to support food and beverage formulation and development efforts, our osmometers help maximize resources and ensure consistent product quality.

Reference

  1. Wesley, J. Sports Hydration: ‘07. 2006 November 2

Comparison Guide

All models support
  • Pharmacopeia testing guidelines

Workflow Features
Best for labs with daily sample processing needs of:>10/day>10/day1-10/day1-10/day
Sample volume100 µL20 µL20 µL250 µL
Sample introductionAutomated liquid handling sample cupSample cupOne-step directSample cup
Operating range (mOsm/kg H2O)0 – 4,0000 – 2,0000 – 2,0000 – 4,000
Test time (seconds)1809090120
Compliance Support Features
Supports 21 CFR part 11 & EU Annex 11 compliance
Supports Pharmacopeia testing guidelines compliance
Instrument Features
Integrated barcode scanner
On-board printer
Multi-language touchscreen display
User name & password compatibility
Multi-sample capacity
Automated cleaning between samples
Automated sample pipetting
Temperature control methodDry coolingDry coolingDry coolingHeat-transfer fluid
Data Management
LIS Connectivity
USB
Webserver
OPC-UA, Network share,
Database backup
DTE RS-232, Barcode port
Part #A2OOsmoTECHPROOsmoTECH3250
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All models support
  • HIPAA, CAP & CLIA compliance

Workflow Features
Best for labs with daily sample processing needs of:>10/day>10/day1-10/day
Sample volume100 µL20 µL20 µL
Sample introductionAutomated liquid handling sample cupSample cupOne-step direct
Operating range (mOsm/kg H2O)0 – 4,0000 – 2,0000 – 2,000
Test time (seconds)1809090
Compliance Support Features
Supports CAP & CLIA compliance
Supports HIPAA compliance
Instrument Features
Integrated barcode scanner
On-board printer
Multi-language touchscreen display
User name & password compatibility
Multi-sample capacity
Automated cleaning between samples
Automated sample pipetting
Temperature control method

Dry cooling

(No heat-transfer fluid)

Dry cooling

(No heat-transfer fluid)

Dry cooling

(No heat-transfer fluid)

Data Management Features
LIS Connectivity
USB
Part #A2OOsmoPROOsmo1
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Services

Get the best performance from your osmometer from the people who designed and built it. We provide a range of services and service plans to meet the different needs of our customers. Our certified Field Service Engineers provide on-site professional support for the installation, training, validation, and maintenance of your osmometers.

Educational Resources

Brochure

A2O Advanced Automated Osmometer

Education

Osmolality in Biopharma

Application Note

Osmolality In Clinical Chemistry