Home Osmometers A₂O Advanced Automated Osmometer

A₂O Advanced Automated Osmometer

5 Reviews

Highlighted Features

Full automation
Gold standard freezing point depression technology
Supports CAP & CLIA compliance

5 Reviews

A₂O Advanced Automated Osmometer

Built to keep the busiest labs productive, the A₂O Advanced Automated Osmometer combines exceptional analytical performance with full automation. Laboratories can depend on the A₂O to deliver accurate and precise osmolality measurements while maximizing walk-away time.

Learn more about COVID-19 and osmolality testing.

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“Have had this over one year and it has needed zero maintenance! As a biomed, I have been very happy with this unit.”

“It's user friendly, which made training relatively simple and reproducibility is very good.”

“The instrument has been reliable and accurate. Advanced Instruments customer service and tech support is outstanding.”

Overview

Industry Applications

IN CLINICAL CHEMISTRY LABS

The premier osmometer for reliable high-throughput osmolality testing of patient samples to drive efficiency for your lab. The A₂O delivers the results security, connectivity, quality control and ease of use to maximize productivity and keep the busiest clinical chemistry labs in compliance.

IN BIOTECH LABS

The full automation of the A₂O keeps your bioprocess development and biologics manufacturing workflows on track with minimum hands-on time. Its exceptional analytical performance helps ensure the highest levels of quality, purity and yield for your biologic product.

IN FOOD AND BEVERAGE LABS

Maximize resources and ensure consistent product quality and process consistency with osmolality testing from formulation to release.

Product Features

AUTOMATED MULTI-SAMPLE CAPABILITY

An intelligently designed 20-position sample tube carousel accommodates the most common laboratory sample tube sizes and shapes. Low volume inserts and nesting cups can also be used.

TOUCH-SCREEN USER INTERFACE

With a menu-driven operating system, intuitive software control, and multi-language capability, the A₂O provides a simple operating experience at your fingertips.

DIRECT PRIMARY TUBE SAMPLING

The instrument automatically transfers 100 µL into the analyzer, eliminating the manual pipetting step for improved accuracy and reduced sampling error.

INTELLIGENT LIQUID HANDLING TECHNOLOGY

The pipette system features liquid level sensing and crash detection circuitry, eliminating the guesswork involved with sample preparation.

AUTOMATED SELF-CLEANING

The closed-loop fluid management system in the A₂O cleans after each sample to eliminate sample carryover and cross contamination.

POSITIVE SAMPLE IDENTIFICATION

Use the integrated bar code scanner to eliminate transcription errors or enter sample IDs manually. The onboard printer provides additional sample identification and results reporting benefits.

FLEXIBLE SAMPLING CAPABILITIES

Program the A₂O to process samples in duplicate, triplicate, or any sampling protocol your laboratory requires.

SELECTABLE SYSTEM OPERATION & ACCESS LEVELS

A₂O system operation and access can be configured in a variety of different ways, depending on the needs of your laboratory. Open access is allowed for any operator to run samples and edit test protocols. A supervisor mode is available that disallows certain operations. Operator login and password protection are also available, allowing the system to associate test results with operator ID.

EASILY INTEGRATE DATA MANAGEMENT INTO YOUR ESTABLISHED WORKFLOW

Quickly save data to a network database, USB device, or the embedded Web server and eliminate the need to manually manage date and time by synchronizing with network time.

DATA MANAGEMENT CAPABILITIES & LIS CONNECTION

The A₂O features an on-board results database with search capability. Connectivity to LIS though ethernet and addtional USB ports.

BUILT IN QUALITY CONTROL

The A₂O comes complete with a full list of QC features including: A supervisor mode with password protection, ability to set range limits for control solutions, statistical monitoring of daily QC, and a system events log for diagnostic purposes.

BACKED BY COMPREHENSIVE SUPPORT

You can purchase any Advanced Instruments osmometer with complete confidence as every instrument is backed by a knowledgeable, experienced, and responsive customer support team.

Technical Specifications

Sample test volume	100μL
Aspirated Sample volume	150μL
Sample capacity	20 sample carousel
Test time	Less than 3 minutes
Sample throughput	can process 20 samples in less than 1 hour
Unit	mOsm/kg H₂O
Calibration	low: 0 to 2000 mOsm/kg H₂O full: 0 to 4000 mOsm/kg H₂O
Resolution	1 mOsm/kg H₂O
Calibrated	3-point Calibrated for low range 4-point for full range
Communication	Onbord printer, 10/100Mbps Ethernet, 4 USB 1.0/1.1/2.0 integrated bar code scanner
Accuracy	0 to 400 mOsm/kg H₂O: mean value ≤ 2 mOsm/kg H₂O from nominal value 400-4000 mOsm: mean value ≤ 0.5% from normal value
Precision (Within-run)	standard deviation ≤ 2 mOsm/kg H₂O between 0 and 400 mOsm; standard deviation ≤ 0.5% of value between 400 to 4000 mOsm
Drift	Less than 1 mOsm/kg H₂O per month
Temperature effects¹	Recalibration recommended for ambient temperature change 5°C (9°F) or greater
Storage temperature	-40°C to +45°C (-40°F to +113°F)
Simple Viscosity	Up to 20 mPa-s(20 cP) higher viscosities may affect pipettor accuracy
Pipettor Accuracy	< 1%
Electrical voltage	100-240 VAC (50/60 Hz)
Power consumption	375W
Dimensions (D x W x H)	20.5″ x 23.6″ x 22.8″ (52 cm x 60 cm x 58 cm)
Net weight	68 lb (31 kg)
Shipping weight	133 lb (60 kg)
Warranty	One-year limited warranty on workmanship and all parts except glass, plastic, and part warrantied by their makers
Operating condition -18°C to 35°C (64°F to 95°F); 5% to 80% relative humidity (noncondensing) specifications subject to change

Associated Products

Supplies & Accessories

200005UG	User’s Guide
200220	Disposable 12×75 mm Sample Test Tubes, Box 250
200221	Probe Wiper Discs, Box 50
200222	Osmometer System Fluid, 1 Bottle 500 mL
200223	Disposable Sample Tubes, Box 500
FLA835	Thermal Printer Paper, 5 rolls

Osmometer Calibration Standards and Reference Solutions

SK-A2O	A2O Convenience Kit for Biopharm/Industry labs includes one each of: the following parts: 200223, 200221, 200222, 3LA028, 3LA201, 3LA301, 3MA029, FLA835, 3LA011, 3LA091
SKC-A2O	A2O Convenience Kit for Clinical Labs includes one each of: the following parts: 200223, 200221, 200222, 3LA028, 3LA201, 3LA301, 3MA029, FLA835, 3LA011, 3LA091, 3MA028, 3LA085
3LA011	100 mOsm/kg H₂O Calibration Standard, 10×5 mL ampules	View
3LA091	900 mOsm/kg H₂O Calibration Standard, 10×5 mL ampules	View
3LA201	2000 mOsm/kg H₂O Calibration Standard, 10×5 mL ampules	View
3LA301	3000 mOsm/kg H₂O Calibration Standard, 10×5 mL ampules	View
3MA029	Clinitrol™ 290 Reference Solution, 10×2 mL ampules	View
3LA028	Osmolality Linearity Set 100-2,000 mOsm 5x2x5 mL	View

Osmometer Control Solutions

3MA028	Protinol 3-Level Osmometer Control 3x3x3 mL	View
3LA085	Renol 2-Level Osmometer Control 2x4x3 mL	View

Clinitrol 290 Reference Solution

10 x 2mL Ampules

View Product

Osmometer Calibration Standards

Available from 0-4000 mOsm/kg H2O 10 x 2mL Ampules

View Product

Osmolality Linearity Set

100, 500, 900, 1500, 2000 mOsm/kg H2O 5x 2x 5mL Ampules

View Product

Applications

Resources for applications of using osmolality within biotechnology processes and manufacturing, clinical chemistry and product quality assurance within food & beverage.

Protein Therapies

When it comes to process development and manufacturing of protein therapeutics, whether you’re making an antibody-based or other protein therapeutic, what you don’t know can slow you down.

Are all of your buffers and media formulated correctly?
Does your solution have unexpected components?
Are all of the steps involving solutions–i.e. cell growth, dialysis, chromatography–proceeding consistently and as expected?

Osmolality, the measurement of total solute concentration, is a reliable control specification and in-process parameter that can deliver information across the protein therapeutic process development and manufacturing workflow.

Upstream

Optimize cell culture conditions to maximize cell density and yield.

Cell Line Development

Ensure that culture conditions are compatible with your clone and cell culture system contributing to optimal cell density and tite.

Media Preparation

Check media composition for optimal cell growth.

Cell Culture Monitoring

Ensure that culture conditions are correct for proper growth and health of cells. This promotes maximum density and yield.

Downstream

Monitor buffer composition and processes, such as chromatography and filtration, to ensure protein purity and yield.

Buffer Preparation

Check for proper buffer composition for efficient processing and optimal quality and yield.

Chromatography

Maintain the purity of the drug intermediates and identify and process deviations that could compromise product yield.

Buffer Exchange

Monitor buffer composition and verify proper exchange to optimize the number of diavolumes and acquire the highest product yield with the highest quality.

Fill & Finish

Monitor and verify the concentration of API and excipients to meet internal quality and regulatory requirements

Formulation Development

Optimize formulation concentration and stability for high yield in manufacturing.

Final Formulation

Monitor and/or verify the concentration of excipients. Confirm soluble content of formulations. Minimize injection site pain.

Filtration and Fill

Key compendial release specification to confirm product stability.

Application Notes

References

Franco R et al. Influence of osmolarity and pH increase to achieve a reduction of monoclonal antibodies aggregates in a production process. Cytotechnology. 1999. 29: 11–25.
Alexandra Steele and Joshua Arias. Accounting for the Donnan Effect in Diafiltration Optimization for High-Concentration UFDF Applications. BioProcess International. Jan 2014. 12(1):50-54.
Wright JF et al. Identification of Factors that Contribute to Recombinant AAV2 Particle Aggregation and Methods to Prevent Its Occurrence during Vector Purification and Formulation. Molecular Therapy. July 2005. 12(1):171-178.
Gonzalez-Pujana A et al. The role of osmolarity adjusting agents in the regulation of encapsulated cell behavior to provide a safer and more predictable delivery of therapeutics. Drug Delivery. 2017. 24(1):1654-1666.
Ohtake S et al. Interactions of formulation excipients with proteins in solution and in the dried state. Adv Drug Deliv Rev. 2011 Oct;63(13):1053-73.
Sahin E et al. Osmolality measurement for high-concentration protein polymer-solutions. BioProcess International. 2016. 14(6):36-43.
Vimpolsek M et al. Assessing the Extended In‐Use Stability of the Infiximab Biosimilar Following Preparation for Intravenous Infusion. Drugs in R&D. 2019. 19:127–140.
Delouvroy F et al. ambrTM Mini-bioreactor as a High-Throughput Tool for Culture Process Development to Accelerate Transfer to Stainless Steel Manufacturing Scale: Comparability Study from Process Performance to Product Quality Attributes. BMC proceedings · December 2015.
Drouillard GC. The Assessment of MAb Quality and Quantity. Masters thesis. University of Guelph. 2015.
El-Enshasy HA et al. Effects of Different Osmotic Pressure of the Cultivation Media on Hybridoma Cell Growth and Monoclonal Antibodies Production Kinetics in Batch Culture. Biotechnology. 2007. 6(2): 202-209.
Konno Y et al. Fucose content of monoclonal antibodies can be controlled by culture medium osmolality for high antibody-dependent cellular cytotoxicity. Cytotechnology. 2012. 64:249–265.
Bishop N et al. Medium Osmolarity Influences Chondrocyte Survival During Photoencapsulation in Poly(ethylene glycol) Hydrogels. 55th Annual Meeting of the Orthopaedic Research Society. 2009. Poster No. 573.
Wucherpfennig T et al. Morphology engineering – Osmolality and its effect on Aspergillus niger morphology and productivity. Microb Cell Fact. 2011 Jul 29;10:58.

Gene Therapy

Consistency is critical when producing genetic therapies, including viral vector delivery and gene editing. Small deviations in the composition of cell growth media can have unwanted effects on cell density, cell composition, and virus yield.

Are you looking for ways to increase vector stability?
Is your gene therapy group looking for higher transfection efficiency and higher viral vector production?

Using osmolality can help your group work towards those goals, as well as answer:

- how to optimize the physiological range for transfection complexes
- identifying media needs
- determining effects of osmotic pressure on the stability of a complex

Upstream

Optimize cell culture conditions and media selection to maximize cell density, size and, ultimately, virus yield.