3250 Single-Sample Osmometer | Advanced Instruments
Single-Sample Osmometer Model 3250
Loading image...

3250 Single-Sample Osmometer

The 3250 Single-Sample Osmometer is designed for routine osmolality measurement in pharma, biotech, and food and beverage labs. Providing accurate and precise osmolality measurement, the 3250 combines proven freezing point technology with the versatility of advanced sample processing capabilities in an osmometer that is both simple to operate and easy to maintain.

Highlighted features:
  • Single-sample osmometer
  • Wide operating range of 0-4000 mOsm
  • Gold-standard freezing point technology
  • Meets Pharmacopeia osmolality testing guidelines

Overview

Industry Applications

biotech

IN BIOTECH LABS

Maximize biologic yield, quality, and purity by optimizing and ensuring the quality and consistency of your buffers, media, product, excipients, and process parameters.

food

IN FOOD & BEVERAGE LABS

Maintain efficiency while ensuring product quality and process consistency with osmolality testing from formulation to release.

PRODUCT FEATURES

GOLD STANDARD PERFORMANCE

The Model 3250 uses a 200-250 μL sample volume and provides the best accuracy and precision of ANY osmometer. Sample test time is 2-3 minutes depending on the operating range

PROVEN RELIABILITY

The 3250 system incorporates over 50 years of applied technology and expertise in the field of osmometry and is ideal for laboratories seeking greater control, minimal downtime, and higher productivity

EASY TO USE

With a push-button menu-driven display, automatic calibration, and onboard statistical analysis, the 3250 bundles world-class performance into a user friendly package

WIDE OPERATING RANGE

The Model 3250 offers the widest measurement range of any osmometer, delivering accurate and precise measurements from 0-4000 mOsm. Select from 2 calibrated operating ranges depending on your sample type

FLEXIBLE REPORTING OPTIONS

The 3250 provides an onboard printer, optional bar code scanner, internal memory storage of test results, and the ability to connect to LIS, data management or middleware such as [email protected] Data Cockpit

BACKED BY COMPREHENSIVE SUPPORT

You can purchase any Advanced Instruments osmometer with complete confidence as every instrument is backed by a knowledgeable, experienced, and responsive customer support team.

Technical Specifications

Sample test volume200 to 250μL
Test timeLow range: 2 minutes (approximate)
High range: 3 minutes (approximate)
Sample capacitysingle sample
UnitmOsm/kg H2O
Resolution1 mOsm/kg H2O
RangeLow range: 0 to 2000 mOsm/kg H2O
High range: 0 to 4000 mOsm/kg H2O
Linearityless than ±0.5% from a straight line over calibrated range
Repeatabilitystandard deviation ≤ 2 mOsm/kg H2O between 0 to 400 mOsm/kg H2O; standard deviation ≤ 0.5% of value between 400 to 4000 mOsm/kg H2O
DriftLess than 1 mOsm/kg H2O per month
Temperature effects1Less than 1 mOsm/kg H2O per 5°C (9°F) ambient temperature change
Calibration• Low range: 0*, 100 and 1500 mOsm/kg H₂O (* optional)
• High range: 1500 and 3000 mOsm/kg H₂O
CommunicationOnbord printer, DTE RS-232 Serial Port and optional bar code scanner
Support LanguagesEnglish, French, German, Spanish, Italian, Portuguese, Swedish, Danish, Turkish, Czech, Slovak
Storage temperature-40°C to +45°C (-40°F to +113°F)
Electrical voltage100 to 240 VAC (50/60 Hz)
Power consumption95W
Dimensions (D x W x H)16″ H x 13″ W x 18″ D (40.6 cm x 33.0 cm x 45.7 cm)
Net weight23.0 lb (10.4 kg)
Shipping weight34.0 lb (15.4 kg)
WarrantyOne-year limited warranty on workmanship and all parts except glass, plastic, and part warrantied by their makers
*operating condition -18°C to 35°C (64°F to 95°F); 5% to 80% relative humidity (noncondensing)
*specifications subject to change

Associated Products

Osmometer Calibration Standards

50, 850 & 2000 mOsm
10 x 2mL Ampules

Osmolality Linearity Set

100-2,000 mOsm
5x 2x 5mL Ampules

Osmometer Calibration Standards and Reference Solutions

SK-3250 BiotechConvenience Kit (Includes: 3LA825, 3DA811, 3LA029, 3LA151, 3LA301, 3LA028, FLA835, 3LA011)
SKC-3250 ClinicalConvenience Kit (Includes: 3LA825, 3DA811, 3LA029, 3LA151, 3LA301, 3LA028, FLA835, 3LA011, 3MA028, 3LA085)
3LA011100 mOsm Calibration Standard, 10×5 mL AmpulesView
3LA029Clinitrol™ 290 Reference Solution, 10×5 mL AmpulesView
3LA091900 mOsm Calibration Standard, 10×5 mL AmpulesView
3LA1511500 mOsm Calibration Standard, 10×5 mLView
3LA3013000 mOsm Calibration Standard, 10×5 mL AmpulesView
3LA028Osmolality Linearity Set 100-2,000 mOsm 5x2x5 mLView

Osmometer Control Solutions

3MA028Protinol 3-Level Osmometer Control, 3x3x3 mLView
3LA085Renol 2-Level Osmometer Control, 2x4x3 mLView

Supplies & Accessories

3LA825Sample Tube, Plastic, Box 500
3DA811Heat Transfer Fluid, 1×150 mL
3255User’s Guide
3255SMService Manual
3D2340Air Filters, Disposable, 6/pkg
330016Barcode Scanner
3LA84630 Sample Tube Rack
FLA835Thermal Printer Paper, 5 rolls

Biotech Services

Get the best performance from your osmometer from the people who designed and built it. We provide a range of services and service plans to meet the different needs of our customers. Our certified Field Service Engineers provide on-site professional support for the installation, training, validation, and maintenance of your osmometers.

Applications

When it comes to process development and manufacturing of protein therapeutics, whether you’re making an antibody-based or other protein therapeutic, what you don’t know can slow you down.

  • Are all of your buffers and media formulated correctly?
  • Does your solution have unexpected components?
  • Are all of the steps involving solutions–i.e. cell growth, dialysis, chromatography–proceeding consistently and as expected?

With the innovative osmometers from Advanced Instruments, you can gain valuable data for managing process and product quality through a simple 90-second test. Osmolality, the measurement of total solute concentration, is a reliable control specification and in-process parameter that can deliver information across the protein therapeutic process development and manufacturing workflow.

See all Advanced Osmometers >

Upstream

Optimize cell culture conditions to maximize cell density and yield.

Cell-Line-Development_TItle

Cell Line Development

Ensure that culture conditions are compatible with your clone and cell culture system contributing to optimal cell density and tite.
Media-Preparation_Title

Media Preparation

Check media composition for optimal cell growth.
Cell-CultureMonitoring_Title

Cell Culture Monitoring

Ensure that culture conditions are correct for proper growth and health of cells. This promotes maximum density and yield.
Downstream

Monitor buffer composition and processes, such as chromatography and filtration, to ensure protein purity and yield.

BufferPreparation_Title

Buffer Preparation

Check for proper buffer composition for efficient processing and optimal quality and yield.
Chromatography_Title

Chromatography

Maintain the purity of the drug intermediates and identify and process deviations that could compromise product yield.
Buffer-Exchange_TItle

Buffer Exchange

Monitor buffer composition and verify proper exchange to optimize the number of diavolumes and acquire the highest product yield with the highest quality.
Fill & Finish

Monitor and verify the concentration of API and excipients to meet internal quality and regulatory requirements

FormulationDevelopment_title

Formulation Development

Optimize formulation concentration and stability for high yield in manufacturing.
Final-Formulation_Title

Final Formulation

Monitor and/or verify the concentration of excipients. Confirm soluble content of formulations. Minimize injection site pain.
Filtration_and_Fill_Title

Filtration and Fill

Key compendial release specification to confirm product stability.

When the therapeutic you’re developing is cell-based, constant monitoring to optimize growth and ensure consistent culture conditions throughout multiple stages is critical for success.

  • Are your stem cells at highest viability and product quality levels throughout culture and passaging?
  • Are you ensuring a high rate of cell recovery post-thaw?
  • Are your transduction mechanics being optimized?

Using osmolality can help your group answer these questions early in the process, ultimately helping you save time, money, and limited or costly reagents. Adding a simple 90-second osmolality measurement to the in-process parameters you measure is a quick and easy way to ensure optimal cell growth, during process development and manufacturing as well as during buffer and media preparation.

With the innovative osmometers from Advanced Instruments, you can keep delicate cells at optimal growth and gain valuable data for managing process and product quality across the cell therapy process development and manufacturing workflow.

See all Advanced Osmometers >

Upstream

Characterize media and optimize transduction of cells with lentivirus to optimize cell viability and size.

Cell-Line-Development_TItle

Cell Line Development

Ensure appropriate trending by monitoring small-scale cell culture for some cell therapies.
Media-Preparation_Title

Media Preparation

Characterize media formulation and QC raw materials for optimal cell growth.
Cell-CultureMonitoring_Title

Cell Culture Monitoring

Ensure that culture conditions are correct for proper growth and health of cells. This promotes maximum cell viability.
Downstream

Optimize downstream processing by confirming buffer concentrations and minimizing shock to cells.

BufferPreparation_Title

Buffer Preparation

Check for proper buffer composition for efficient processing and optimal quality and yield.
Chromatography_Title

Chromatography

Maintain purification conditions to maximize yield and protect cells.
Buffer-Exchange_TItle

Buffer Exchange

Optimize solution concentrations for UF/DF concentration and buffer exchange to acquire the highest product yield with the highest quality.
Fill & Finish

Monitor and ensure final composition and manage cryopreservation and storage.

FormulationDevelopment_title

Formulation Development

Optimize formulation concentration and composition drug safety.
Final-Formulation_Title

Final Formulation

Confirm concentration of formulations and ensure compatibility of cells with cryopreservatives.
Filtration_and_Fill_Title

Formulation and Fill

Key compendial release specification to confirm stability.

Consistency is critical when producing genetic therapies, including viral vector delivery and gene editing.  Small deviations in the composition of cell growth media can have unwanted effects on cell density, cell composition, and virus yield.

  • Are you looking for ways to increase vector stability?
  • Is your gene therapy group looking for higher transfection efficiency and higher viral vector production?

Using osmolality can help your group work towards those goals, as well as answer:

    • how to optimize the physiological range for transfection complexes
    • identifying media needs
    • determining effects of osmotic pressure on the stability of a complex

With the innovative osmometers from Advanced Instruments, you can gain valuable data for managing process and product quality through a simple 90-second test. When done right, osmolality, the measurement of total solute concentration, is a reliable control specification and in-process parameter that can deliver information across the gene therapy process development and manufacturing workflow.

See all Advanced Osmometers >

Upstream

Optimize cell culture conditions and media selection to maximize cell density, size and, ultimately, virus yield.

Cell-Line-Development_TItle

Cell Line Development

Ensure that culture conditions are compatible with your clone and cell culture system, contributing to optimal cell density and titer. Maximize transfection efficiency for optimal yield.
Media-Preparation_Title

Media Preparation

Characterize media preparation and composition for optimal cell growth.
Cell-CultureMonitoring_Title

Cell Culture Monitoring

Ensure that culture conditions are correct for proper growth and health of cells. This promotes maximum cell density and yield.
Downstream

Check raw material concentration and perform in-process monitoring of purification and filtration steps to maximize purity, cell/virus stability, and yield.

BufferPreparation_Title

Buffer Preparation

Check for proper buffer composition for efficient processing and optimal quality and yield.
Chromatography_Title

Chromatography

Maintain the purity of the drug intermediates, protect the viral vector and genetic content within the vector during process, and identify process deviations that could compromise product yield.
Buffer-Exchange_TItle

Buffer Exchange

Monitor buffer composition and verify proper exchange to optimize the number of diavolumes and acquire the highest product yield with the highest quality.
Fill & Finish

Monitor and ensure final composition, dosage, and stability

FormulationDevelopment_title

Formulation Development

Optimize formulation concentration and composition for high yield in manufacturing.
Final-Formulation_Title

Final Formulation

Monitor and/or verify the concentration of excipients. Confirm soluble content (solute concentrations) of formulations. Ensure compatibility of precious product with cryopreservatives.
Filtration_and_Fill_Title

Filtration and Fill

Key compendial release specification to confirm product dosage specifications.

Osmolality testing has unlimited applications in areas of personal care and hygiene. Products that come into contact with or penetrate the skin must have an osmolality within a physiological range to avoid damaging cells and tissues. In some instances, slightly hypo- or hyper-osmotic solutions are generated to promote wetting or drying effects. The manufacturing of skincare products, personal lubricants and optical products

One key to a youthful look is healthy and moisturized skin. Proper hydration of skin depends on a balance of water flow into and out of skin cells and, thus, depends on osmolality. Osmolality testing of skincare products provides assurance that the product will have moisturizing effects and serves as a control during the production process.

The osmolality of personal lubricant formulations impacts their effect and safety. The World Health Organization (WHO) stated that the osmolality of these solutions should not exceed 1200 mOsm/kg, with this limit decreasing to a more iso-osmotic level in the future.

Similar value for osmolality testing can be clearly seen in optical applications. Eye drops and other optical products are designed to lubricate or protect the eye. The goal of these solutions (e.g. adding moisture, removing moisture, etc.) dictates an ideal osmolality range.

See all Advanced Osmometers >

References

  • Aragona P et al. Physiochemical Properties of Hyaluronic Acid-Based Lubricant Eye Drops. Trans Vis Sci Tech. 2019;8(6):2.
  • Corrales R et al. Effects of Osmoprotectants on Hyperosmolar Stress in Cultured Human Corneal Epithelial Cells. Cornea 2008;27:574-579.
  • WHO/UNFPA/FHI, 2011-. Advisory Committee Notes to the Female Condom Technical Review Committee Meeting. [Geneva, Switzerland].

Osmolality testing is required to ensure that the final vaccine product is safe for administration. The osmolality must be within a physiological range to be introduced in human tissues. There is also evidence that injectable formulations with high osmolality may cause pain at the site of injection.

Vaccines are derived from a variety of sources, both living and synthetic, meaning a variety of bioproduction workflows are required. These workflows include some common use cases for osmolality testing, including the following:

  • Upstream cell culture and/or cell expansion requires maintaining an environment at the appropriate osmolality for the specific cell type. For those modalities that are cell-based, regular osmolality testing will ensure that the media and environment are optimized.
  • Buffers used during any downstream processing should be tested for osmolality to verify the concentrations of the solutions that are meant to protect the drug substance throughout the chromatography and/or filtration operations.
  • Final product testing must include osmolality to verify identify and safety of any injectable.

Differentiation in use cases for osmolality testing are outlined below:

Monoclonal Antibody Vaccines

Osmolality testing should be performed on hybridoma cell media to ensure optimal antibody production rates.

  • The rate of antibody production in hybridoma cells depends on the osmolality of the medium in which the cells are grown.
  • The osmotic agent used to affect the osmolality levels also impacts Ab production.

Viral Vaccines

Osmolality testing should be performed on media solutions to optimize cell size and viral vector stability.

  • Osmotic pressure affects the size of the morphology of insect cells and viruses.
  • The osmolality of the media affects the composition of the lipid bilayer membrane of enveloped viruses. This directly impacts the vector stability.

mRNA and DNA Vaccines

Delivery systems and mechanisms should be tested for osmolality.

  • mRNA can be complexed with different agents (e.g. PEI) for delivery within the body. Osmolality impacts the stability of the complex of viral models.
  • Osmolality impacts the ability to penetrate the target cell membrane.

COVID-19 vaccine development often falls into these development categories. To better understand how osmolality testing benefits the production of therapies, visit the respective tabs: Protein Therapies, Cell Therapies, Gene Therapies, Oligonucleotides.

See all Advanced Osmometers >

An example of an accelerated mRNA vaccine production workflow is below:

Accelerated Development
(as little as 1 year)

References

This novel field involves the use of antisense oligonucleotides (ASOs), aptamers, siRNAs, miRNAs and synthetic mRNAs to control the expression of genes that have been associated with diseases. Multiple Advanced Instruments customers within this field use osmometers to verify the concentration of the solutions carrying their precious products. Ultimately, the quality of the drug is dependent on the process controls implemented throughout the workflow. Below are some of the current uses for osmolality testing in this field:

Buffer QC

Osmolality specifications for buffers to preserve and protect the critical quality attributes of the product as it is being developed.

Final Product

Compendial release test needed to confirm the identity and concentration of the oligo product for safe use.

Because this is an emerging field, it comes with a number of development and application challenges:

  • Delivery of RNAs across the lipid bilayer and into cells
  • Immunogenicity once in the body
  • High rates of degradation of oligos by plasma and tissues

Drug delivery into the target cells requires the proper osmotic pressure, similar to the parameters around viral transfection in vector development. The optimal osmolality of the delivery complex (e.g. mRNA:PEI) should be determined and monitored during this process. This specification will provide repeatable successful delivery of the drug.

See all Advanced Osmometers >

References

Comparison Guide

All models support

Pharmacopeia testing guidelines

Being discontinued Dec. 31, 2020
Workflow Features
Best for labs with daily sample processing needs of:>10/day>10/day1-10/day1-10/day1-10/day
Sample volume100 µL30 µL20 µL20 µL250 µL
Sample introductionAutomated liquid handling sample cup (automated)Sample cupOne-step directOne-step directSample cup
Operating range (mOsm/kg H2O)0 – 4,0000 – 2,0000 – 4,0000 – 2,0000 – 4,000
Test time (seconds)18090Less than 150 in low range, Less than 180 in high range90120
Compliance Support Features
Supports 21 CFR Part 11 & EU Annex 11 compliance
Supports Pharmacopeia testing guidelines compliance
Instrument Features
Integrated barcode scanner
Printer
Multi-language touchscreen display
User name & password compatibility
Multi-sample capacity
Automated cleaning between samples
Automated sample pipetting
Temperature control methodDry cooling (No heat transfer fluid)Dry cooling (No heat transfer fluid)Dry cooling (No heat transfer fluid)Dry cooling (No heat transfer fluid)Heat-transfer fluid
Data Management Features
TCP/IP
USB
Webserver
OPC-UA, Network share,
Database backup
DTE RS-232, Barcode port
Part #A2OOsmoTECHPROOsmoTECHXTOsmoTECH3250
Download Chart as a PDF

  1. Sold separately
  2. Printer (dot-matrix) sold separately
Learn More
Request Pricing
Learn More
Request Pricing
Learn More
Request Pricing
Learn More
Request Pricing
Learn More
Request Pricing

Educational Resources

Brochure

Advanced 3250 Single Sample Osmometer

Education

Osmolality as a concentration measurement method for key buffers in bioprocessing

Application Note

Osmolality as a predictor for model-based real-time monitoring of concentration in protein chromatography

We are open and continue to provide essential lab supplies during the COVID-19 pandemic. Answers to your FAQ’s can be found here.

Read More
X