Oligonucleotide Manufacturing | Advanced Instruments


Verifying the concentrations of your solutions during oligonucleotide manufacturing.

Emerging Uses for Osmolality

This novel field involves the use of antisense oligonucleotides (ASOs), aptamers, siRNAs, miRNAs and synthetic mRNAs to control the expression of genes that have been associated with diseases. Multiple Advanced Instruments customers within this field use osmometers to verify the concentration of the solutions carrying their precious products.Ultimately, the quality of the drug is dependent on the process controls implemented throughout the workflow.

Supporting Innovative Technology

Because oglio manufacturing is an emerging field, it comes with a number of development and application challenges:

  • Delivery of RNAs across the lipid bilayer and into cells
  • Immunogenicity once in the body
  • High rates of degradation of oligos by plasma and tissues

Drug delivery into the target cells requires the proper osmotic pressure, similar to the parameters around viral transfection in vector development. The optimal osmolality of the delivery complex (e.g. mRNA:PEI) should be determined and monitored during this process. This specification will provide repeatable successful delivery of the drug.


Current Applications of Osmolality in Oligonucleotide Manufacturing and Development

As the field of oligonucleotides continues to develop the uses of osmolality continue to grow. Below are some of the current uses for osmolality testing in this field:

The system has excellent accuracy and precision, even between different analysts. The great thing about the XT model is that it can measure a broad range of sample osmolality to cover all of our sample needs.

Will Heaton, DiscGenics

Popular Educational Resources

Learn more about how our osmometers can be utilized to optimize your workflows by checking out our educational resources below


Impact Of Osmolality During The AAV Manufacturing Workflow


8 Ways That Osmolality Testing Improves Cell and Gene Therapy Processing


Osmolality Measurement of Lentiviral Vectors for Cell and Gene Therapy

Associated Products

OsmoTECH® XT Single-Sample Micro-Osmometer

Best-in-class osmolality performance, designed for the toughest samples

OsmoTECH® PRO Multi-Sample Micro-Osmometer

Combines ease-of-use with accurate and precise measurements for reliable results


Bioprocessing Services

Get the best performance from your osmometer from the people who designed and built it. We provide a range of services and service plans to meet the different needs of our customers.

Our certified Field Service Engineers provide on-site professional support for the installation, training, validation, and maintenance of your osmometers.