Employing Tools of ICON to Advance Selection of the Highest Producing Clones
Speaker: Jenica Lee, Senior Research Associate, Wheeler Bio
Presented as part of X-Talks: Optimal Cell Line Development: ICON and STUDIUS as part of the whole Ecosystem including VIPS and Cell Metric June 6, 2023
Duration: ~10 min
In cell line development today, the pressure to make decisions on best-performing clones earlier and shorten timelines continues to grow. One factor in the decision-making process is a cell line’s productivity, necessitating the measurement of titer.
Currently, titer measurements in cell line development are mostly performed using incumbent methods such as Octet and ELISA, which have their strengths but were not specifically developed for cell line development applications. The minimum sample volume requirements of the incumbent methods mean that titer measurements have been neglected at the earlier cloning plate stage when 96 and 384 well plates are being used. Alternatively, samples have been diluted, leading to additional liquid handling steps and possible errors. During this webinar, attendees will see featured case studies from users of the Solentim ICON, an instrument that was specifically designed to meet the demands of the cell line development workflow. ICON technology uses lower sample volumes for titer and viable cell density (VCD) measurements in higher density formats such as 384, 96 and 24 well plates. The case studies also show how faster titer measurements helped to allow clones to be excluded more rapidly, enabling decision-making earlier and shortening the cell line development process by days to weeks.
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