Our portfolio of instruments, consumables and services are designed to help you maintain regulatory guidelines, ensure data integrity, and meet GMP requirements.
Our portfolio of instruments, consumables and services are designed to support clinical lab regulations, standards and with HIPAA data security in mind.
Maximizing Viral Vector Titer With Osmolality Measurement
Viral vector titer is highly dependent on vector stability, which is inherently compromised in retroviruses by the lipid bilayer envelope. Various publications show evidence that vector stability, particularly for enveloped vectors, improves at higher media osmolality. This is due to the effects of media osmolality on lipid production and the final composition of the membrane. Using osmolality testing is a valuable analytic during viral transfection. We will discuss data that supports the implementation of osmolality specifications for cell media, as process repeatability and product yield depend on this process control.
Viral vector titer is highly dependent on vector stability, which is inherently compromised in retroviruses by the lipid bilayer envelope. Various publications show evidence that vector stability, particularly for enveloped vectors, improves at higher media osmolality. This is due to effects of media osmolality on lipid production and the final composition of the membrane. Using osmolality testing is a valuable analytic during viral transfection. We will discuss data that supports the implementation of osmolality specifications for cell media, as process repeatability and product yield depend on this process control.