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Viral vector titer is highly dependent on vector stability, which is inherently compromised in retroviruses by the lipid bilayer envelope. Various publications show evidence that vector stability, particularly for enveloped vectors, improves at higher media osmolality. This is due to effects of media osmolality on lipid production and the final composition of the membrane. Using osmolality testing is a valuable analytic during viral transfection. We will discuss data that supports the implementation of osmolality specifications for cell media, as process repeatability and product yield depend on this process control.