Osmolality is a measurement of the total number of solutes in a liquid solution expressed in osmoles of solute particles per kilogram of solvent (osmol/kg).
Living cells are often sensitive to changes in osmolality, as water can rush in or out of their porous cell membranes in response, causing cells to burst or shrink. Therefore, testing osmolality of various solutions enables assessment of cell functioning and volume. This is relevant in two different settings:
From the development of a biopharmaceutical drug to the final product formulation and fill, bioprocessing involves many complex operational steps. Ensuring that this process consistently generates a solution of predetermined yield and purity is a key factor for the highest quality in the final product.
An important parameter to achieve consistent results in process fluids is the solute concentration. As a fast, easy to implement and reliable measure of concentration, osmolality is therefore considered a critical quality attribute and critical process parameter in bioprocessing.
Clinical labs test osmolality of serum, plasma and urine because it provides information on the body’s water and electrolyte balance. Abnormal osmolality levels are typically an indicator of a serious homeostatic imbalance, often due to renal dysfunction, diabetes, infection or poisoning. For this reason, measuring osmolality is critical when you suspect toxin ingestion, electrolyte disorders and metabolic acidosis.
Osmolality is measured using a device called an osmometer. These instruments measure osmotic concentration on the scale of milliosmoles (mOsm) per unit of weight (mOsm/kg): This means they operate on an order of a thousand times smaller than an osmole.
Freezing point osmometers are commonly used to determine osmolality.
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