FLM300 Fluorophos ALP Test System

The most sensitive pasteurization determination

Take phosphatase testing to its highest level

The Fluorophos ALP assay is a rapid test for verifying proper pasteurization of dairy products. It takes phosphatase testing to its highest level by yielding quantitative results and eliminating subjective interpretation. A versatile system for dairy products, the Fluorophos ALP Test System can test products made from cow, goat, sheep, and other species.

Highlighted features
  • Fast & accurate results
  • Globally proven
  • Reduced production liability

The most sensitive pasteurization determination

Alkaline Phosphatase (ALP), found naturally in raw milk, is destroyed at temperatures slightly above the temperature necessary to destroy microorganisms pathogenic to humans.

Measurement of residual phosphatase after pasteurization offers a means of verifying the products are heated to the proper temperatures and are not contaminated with raw milk.

The Fluorophos ALP assay measures the ALP activity in dairy products, allowing the user to monitor the completeness of pasteurization and detect raw milk contamination.

Flexible Consumables

Easier and More Reliable

PhosphaCheck Pasteurization Controls allow you to monitor your assay. These consistent, reliable, off-the-shelf controls readily replace in-house manufactured controls.


With this set of positive, negative, and normal PhosphaCheck Controls, you are assured quantitative levels of ALP—every time.


  • Reliable controls for milk and dairy prodcuts offering quantitative levels of alkaline phosphatase (ALP)
  • Specifically designed to satisfy all QC and HACCP requirements
  • Monitor the precision of your lab pasteurization test
  • Designed for use with NCIMS Form 2400j-1
  • Use to meet local regulatory procedural requirements

Product Features

  • Results in just 3 minutes

  • ISO/IDF Reference method
  • Standard for confirming proper pasteurization for NCIMS/FDA, AOAC, IMS, and CEN

  • Detects to 0.0003% raw milk contamination

  • Test pasteurization of cow, goat, sheep, and other milk species

  • Single sample test system

  • Detects ultra-low levels of raw milk contamination


Yes, the phosphatase test using the Fluorophos ALP Test System is a recognized reference method per ISO/IDF 11816-1, FDA 2400, Bacterial Analytical Manual (BAM), and AOAC Section 979.13.

Electrical power requirement 100 to 240 VAC (50/60 Hz)
fuses (2) 250V time deley (type T): 1 Amp
Power consumption 180 Watts
Mwmory backup Integral lithium cell; 10 year life (typical); not user replaceable
Sample test volume See test kit instruction
Aspirated sample volume 150 μL
Sample capacity Single sample
Readout 20 Character vacuum florescent display
Unit FLU (florescence Unit)
Result Unit See testkit instructions
Zeroing Automatic
Resolution 1 FLU
Drift Less Than 3 FLU per hour
Cuvette size 12 X 75 mm round
Warmup time Cuvette chamber 15 minutes, Heating block 30 minutes
Test time 3 minutes
Optical 90 Optical bench with light source, fixed filter, detector
light source Light Emitting diode
Filter Excitation 440 nm bandpass, Emission 550 nm longpass
Storage temperature -40 to 113° F/-40 to 45 °C
Operating temperature 68 to 85° F / 20 to 29 °C
Room humidity 5-80% relative humidity (non-condensing)
sound level 71.5 dB(A) maximum at operator’s option
  • integrated thermal printer
  • DTE EIA-232/V.24(RS-232) serial port (boud Selectable 1200, 2400, 4800, 9600 or 19200)
  • Optional barcode reader
Dimensions (D x W x H) 6.5″ x 12.5″ x 16.5″ (17cm x 32 cm x 42 cm)
Net weight 18 lbs. (8 kg)
Shipping weight 34 lbs. (15 kg)
IEC protection class I
Over-voltage category II
Pollution degree I2
Moisture protection IPXO (ordinary)

ALP Pasteurizing Controls

FLMA02Reagent Kit (Includes: FLA224, FLA250, FLA260, FLA020)
FLA005Fluorophos Cheese Buffer
FLA224ALP 225-Test Kit/No Cuvettes
FLA225ALP 225-Test Kit/With Cuvettes
FLA250ALP Three Calibrator Set
FLA260Phosphacheck Milk ALP Controls
FLA280ALP Daily Instrument Control


SK-FLM300Convenience Kit (Includes: FLA880, ACM205, FLA840, FLA835)
ACM205Pipette Tips for FLA888, 2 mL- 25 pack
FLA835Thermal Printer Paper, 5 rolls
FLA840Cleaners for Cuvette Holder
FLA86525 µl Pipette
FLA880Pipette Tips, 25- 75 µl
FLA8882 mL Air Displacement Pipette

Food & Beverage Services

Installation and Training
Expert installation and training, available only from Advanced Instruments.

Advanced Care Service Agreements
Get total peace of mind with a service agreement—to ensure protection in case of instrument downtime.

Learn more
Application Note

Control of Pasteurized Milk

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Knowledge Center

Alkaline Phosphatase Testing For Pasteurized Milk

Learn More
Knowledge Center

Dairy Milk Analysis

Learn More

Request pricing

For a quotation, to arrange a demonstration, or for more information please contact us by phone or complete the form.

Austria: +49 695 0606 800
Belgium: +31 6 899 25902
France: +33 456 681 861
Germany: +49 695 0606 800
The Netherlands: +31 6 899 25902
United Kingdom: +44 740 890 1366

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Please fill out this brief form to download this content. You can also get in touch with our sales team for product questions, arranging a product demonstration or obtaining a quotation at the phone numbers below:

Austria: +49 695 0606 800
Belgium: +31 6 899 25902
France: +33 456 681 861
Germany: +49 695 0606 800
The Netherlands: +31 6 899 25902
United Kingdom: +44 740 890 1366