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Solentim iPSC Matrices: MatriClone™ and Clinical Manufacturing Grade Laminin

Support iPSC growth and pluripotency in both single and multi-cell formats
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High viability iPSC single cell cloning and outgrowth

MatriClone is a new matrix designed to support iPSC growth in single seeding workflows while maintaining pluripotency. MatriClone is optimized for ‘in-solution’ seeding and well suited for automated imaging. When used as part of the VIPS PRO seeding protocol, MatriClone ensures high levels of single cell survival and subsequent outgrowth. This matrix simplifies the workflow by eliminating the need to coat plates. MatriClone is composed of a defined matrix containing a recombinant peptide and is animal component-free.

Highlighted features
  • Higher process efficiency
  • Promotes pluripotency
  • Optically suited for automated imaging
  • Compatible with VIPS PRO
  • Support iPSC growth in single and multi-cell formats

Combine Solentim iPSC Matrices with VIPS PRO to enhance iPSC cloning

MatriClone and Clinical Manufacturing Grade Laminin are capable of supporting iPSC growth and pluripotency in both single and multi-cell formats.

As the matrices are both soluble, and optically suited for automated imaging, the matrices can be paired with Advanced Instruments VIPS to improve cloning efficiency.

 

MatriClone and Clinical Grade Laminin Calculator

In-Solution Pre-Coating
Well surface area
Required Matrix coating density
µg/cm²
The standard optimum coating density is 0.25 μg/cm², however MatriClone In-Solution can be between 0.1 and 1.5 μg/cm².
The standard optimum coating density is 0.5 μg/cm², however MatriClone In-Solution can be between 0.1 and 1.5 μg/cm².
Number of wells to coat
Submit

Use our calculator to help you work how much matrix you require

Our matrices can be used both in-solution, or to pre-coat plates. The term ‘in-solution’ refers to adding the matrix to the culture media at the same time as the cells during cell passaging, removing the need to pre-coat plates. The optimum coating density for both in-solution and pre-coating methods may differ depending on the cell line, application, and medium selected but don’t worry, our useful calculator is here to help! Select if you will be using in-solution or precoating, your surface area and number of plate to find out how much product of each matrix is required.

When it comes to using MatriClone or Clinical Manufacturing Grade laminin in-solution, the important thing to remember is that the required volume of matrix is independent of the well fill volume, it’s all about surface area.

For example:

  • To coat a 6-well plate (9.6 cm2/well) with 0.25 μg/cm2 following the in-solution method, 4.8 μL of MatriClone is required per well.
  • To coat all 6 wells in the plate, 28.8 μL of MatriClone should be added to 12 mL of cell suspension before seeding 2 mL of cell suspension, plus MatriClone, into each well.
  • Should the scientist decide to fill each well with 1.5 mL of medium instead, the quantity of MatriClone would still be 4.8 uL per well because the surface area of the well has not changed.

Supports Regulatory Approval

MatriClone and Clinical Manufacturing Grade Laminin are optically suited for whole well imaging, allowing scientists to capture crucial whole well images required for regulatory approval. The matrices, combined with the Cell Metric X and VIPS PRO technologies, enable extremely highest resolution images to be captured ready for submission.

Improved iPSC Adhesion

Laminin-511 is a major component of the basement membrane which is expressed in early development of the embryo. There are numerous forms of Laminin, all of which promote iPSC growth; however, this truncated version of the recombinant Laminin-511 promotes greater adhesion than full-length Laminin protein, in addition to other market alternatives.

Supports iPSC Pluripotency

MatriClone and Clinical Grade Laminin are comprised of the laminin 511 isoform which is instrumental to the growth and maintenance of iPSCs. Immunohistochemistry (IHC) staining has been used to confirmed the expression of important pluripotent markers: OCT4 and NANOG.

FAQs

MatriClone™ is a xeno-free, recombinant Laminin-511 E8 fragment produced in CHO-S cells which is designed to support the early growth of Induced Pluripotent Stem Cells (iPSCs). MatriClone supports iPSC growth and pluripotency in both single and multi-cell formats and and is optically suited for automated imaging.

Absolutely! Advanced instruments are uniquely positioned to provide both research grade MatriClone, and Clinical Manufacturing Grade Laminin from the same source material, ensuring quality and consistency across the research and GMP iPSC workflows.*

*To purchase Clinical Manufacturing Grade Laminin, you must have Solentim instrumentation in your lab. 

MatriClone and Clinical Manufacturing Grade Laminin must be used in combination with tissue culture treated plates.

The term ‘in-solution’ refers to adding the matrix to the culture media at the same time as the cells during cell passaging. As MatriClone and Clinical Manufacturing Grade Laminin are soluble, there is the option to add it during plating rather than pre-coating the plates.

Using MatriClone/Clinical Manufacturing Grade Laminin in-solution saves time as you no longer need to coat your plates ahead. Simply add the matrix to your cell suspension and seed directly into a tissue culture treated plate!

Additionally, in-solution use requires much less matrix compared to pre-coating.

Please note that MatriClone/Clinical Manufacturing Grade Laminin must be used in-solution when seeding with Advanced Instrument’s VIPS PRO.

Yes, however this process is very simple. Once cells have reached confluence and are ready to be passaged, simply dissociate them following your standard protocol. Instead of plating the cells onto your traditional matrix, seed your cells into a plate which has been pre-coated with MatriClone/Clinical Manufacturing Grade Laminin, or add MatriClone/Clinical Manufacturing Grade Laminin during plating (if following the in-solution protocol).

Cells can be dissociated using any of the traditional dissociation reagents. Internally, we have experience with Accutase, TrypLE, Versene, ReLeaSR, GDCR and EDTA. We have found TrypLE and ReLeaSR to be the most popular amongst our customer base.

Yes, the matrices are suitable for clump and single cell passaging of iPSCs.

MatriClone, is always supplied in 175 µg vials. Customers can purchase MatriClone in packs of 2 or 6.

Please email [email protected] if you have any further questions.

RS-2100MatriClone – 2 x 175µg
RS-2101MatriClone – 6 x 175µg
RS-4100Clinical manufacturing grade laminin is now available for customers to use in combination with Solentim instrumentation.
Solentim VIPS® PRO
High efficiency, single cell seeding and imaging platform

High efficiency, single cell seeding with image-based proof of clonality for GMP-compatible workflows

Learn more
Solentim Cell Metric® X
Clonal imager with automated assurance

Simplify clonal assurance with Artificial Intelligence

Learn more
Insti Cell Growth Reagents

Cell growth supplements which improve cell growth and accelerate cell line development workflows for CHO and HEK cells.

Learn more

Services

Offering a wide range of services including Advanced Care service agreements, Installation & Training packages and Validation reports, we keep customers productive and their results accurate, with top-level support at and beyond their purchase.

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MatriClone and clinical grade laminin are the perfect choice for:

  • A seamless transition from process development to manufacturing
  • Automating of iPSC cloning, in combination with VIPS PRO
  • Confidence in regulatory submissions
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Establishing New Standards in Efficient Single Cell Cloning of hiPSCs

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Application Note

High efficiency single cell cloning of hiPSCs whilst ensuring maintenance of phenotype

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Application Note

Development of a highly efficient, documented workflow for making clonal cell banks of gene-edited iPSCs

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For a quotation, to arrange a demonstration, or for more information please contact us by phone or complete the form.

Austria: +49 695 0606 800
Belgium: +31 6 899 25902
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Germany: +49 695 0606 800
The Netherlands: +31 6 899 25902
United Kingdom: +44 740 890 1366

Download Brochure

Please fill out this brief form to download this content. You can also get in touch with our sales team for product questions, arranging a product demonstration or obtaining a quotation at the phone numbers below:

Austria: +49 695 0606 800
Belgium: +31 6 899 25902
France: +33 456 681 861
Germany: +49 695 0606 800
The Netherlands: +31 6 899 25902
United Kingdom: +44 740 890 1366