Cell Growth Supplements

Enhancing Cell Line Development Workflows

High performance cell growth supplements

The demand for protein therapeutics and novel gene therapies has soared since their introduction, driving the need for improved production methods which ensure both higher capacity and consistent product quality. However, development remains a challenge due to numerous limitations in cell culture practices.

Advanced Instruments proudly offers a suite of animal component free (ACF) cell growth supplements tailored for Chinese Hamster Ovary (CHO) cells and Human Embryonic Kidney Cells (HEK). The InstiTHAW™, InstiGRO, and InstiSHAKE™ cell culture supplements have been carefully crafted to protect, nurture, and support these cell lines to deliver unparalleled growth and performance.

Highlighted features
  • Designed for HEK and CHO
  • Improve cloning efficiency
  • Animal Free Certification
  • Reduce CLD Project Timelines
  • Manufactured under ISO 9001
  • Ready to use

The Key to Success

Ensuring that cells receive the metabolites they need is fundamental for success. While cell culture mediums do their best, cells often need a little extra support from cell culture supplements to overcome stressful processes such as cryopreservation, revival, single cell isolation, and expansion to larger shaking vessels.

While InstiTHAW protects cells during cryopreservation and revival, InstiGRO assists with early cell growth to promote the formation of healthy clonal colonies. Lastly, InstiSHAKE supports the transition of cells from static culture to shaking culture conditions, paving the way for scalable production of protein therapeutics and gene therapies.

InstiTHAW

  • Improves host cell revival – Allows cell line development campaigns to commence with hast following host cell revival.
  • Instills confidence in the health of the master cell banks – Protects cells during cryopreservation and revival, providing assurance when it comes to generating master cell banks.
  • Easy to use – Simply add to your cell suspension during cryopreservation, and at the time of revival.

    InstiGRO

    • Enhances clonal outgrowth – Provides single cells with the crucial nutrients required to develop into healthy clonal colonies.
    • Compatible with whole well imaging for regulatory approval – Capturing high resolution images for Investigational New Drug applications is crucial. InstiGRO promotes single cell growth whilst preserving optical clarity.
    • Easy to use – Simply add to your cloning medium before single cell seeding.

    InstiSHAKE

    • Supports clonal expansion – Protects cells as they transition from static to shaking culture vessels, enabling scalable manufacture.
    • Nourishes the health of bulk cultures – Nurtures the viability and viable cell density of cells in shaking culture vessels.
    • Easy to use – Simply add to your cell culture medium.

    Customer Experiences

    “We use InstiGRO CHO to improve the efficiency of our cloning projects. The supplement has helped us achieve clonal outgrowth >80% and has reduced the time taken for clones to reach our desired confluence by 2 weeks!

    During expansion, we use InstiSHAKE CHO to support the adaptation of our clones to shaking culture. Thanks to InstiSHAKE CHO, we have significantly reduced the number of clones lost (by 90%) and have reduced the amount of time taken for cells to reach our desired viable cell density and viability by 7 days.

    Taken together, the combined use of InstiGRO CHO and InstiSHAKE CHO can accelerate our CLD workflow up to 3 weeks…”

    Stable or Transient Development, We’ve Got You Covered

    InstiGRO + VIPS PRO: Unlocking Potential

    VIPS PRO is an automated cell seeder and whole well imaging system which dispenses single cells and captures high-quality whole well images to provide assurance of clonality. The system enhances single seeding efficiencies and is pivotal in supporting Investigational New Drug applications.

    Pairing VIPS PRO’s high throughput seeding and imaging capabilities with InstiGRO’s ability to support single cell outgrowth unlocks a new level of potential. The result? Reduced project costs and timelines, empowering scientists to achieve their goals more efficiently than ever before.

     

    *InstiGRO HEK, InstiSHAKE HEK and InstiTHAW HEK products are classified as animal component-free (ACF), and chemically defined (CD).  Advanced Instruments relies on supplier-provided documentation for information related to ingredient origin, composition, and classification.  Not all information may be known beyond the ingredient level. 

    FAQs

    Once opened, the cell culture supplements are viable for the full shelf life specified on the Certificate of Analysis.

    No, none of the cell culture supplements are sensitive to light exposure.

    No, filtering the cell culture supplements will not affect their performance. All cell culture supplements are supplied sterile so there is no need to filter them in this respect. However, if you are using InstiGRO for single cell cloning, we do recommend that you filter your cloning medium + InstiGRO mix before starting single cell seeding.

    All our cell culture supplements are manufactured at our facility in the Southwest of the United Kingdom.

    No, manufacturing grade InstiGRO, InstiSHAKE, and InstiTHAW are not commercially available at present. To help us understand your needs further, please contact your local representative from Advanced Instruments, or email us at [email protected].

    While the CHO and HEK supplements have been designed and validated for suspension CHO and HEK cell lines, we have seen great results both internally and amongst our academic customer basis with adherent cell lines. For more information, please email us at [email protected].

    No, each batch of cell culture supplements is subject to stringent quality control checks to ensure that there is no lot-to-lot variability.

     

    InstiGRO CHO, InstiGRO CHO PLUS, and InstiGRO HEK are all available in both 5 mL and 25 mL quantities. InstiSHAKE CHO and HEK are only available in 25 mL quantities, while InstiTHAW CHO and HEK are only available in 5 mL quantities.

    No, simply add InstiGRO to your standard outgrowth medium, including any other additives or selective agents which are usually used.

    While InstiGRO will continue to support the cells as they progress through static culture expansion, there is no need to keep adding the supplement if your clones are established and are growing well. Simply expand cells to the next culture vessel in their standard cell culture medium. There is no need to gradually ween the cells from InstiGRO’s support.

    Yes, as detailed on the Instructions for Use (IFU) document, cells should be cultured with a 1X concentration of InstiGRO for 4-24 hours prior to single cell seeding.

    No, simply add InstiSHAKE to your standard medium, including any other additives or selective agents which are usually used.

    While InstiGRO CHO and CHO PLUS are both designed to support clonal outgrowth, the formulations are slightly different to cater for different cell lines and growth mediums. It is often difficult to predict which formulation will work best. We therefore recommend that all new customers evaluate clonal outgrowth with both formulations before deciding how to proceed.

    No, InstiTHAW does not replace DMSO. InstiTHAW should be considered as an additional supplement to your standard cryoprotective solution.

    Please email [email protected] if you have any further questions.

    Support the development of biologics with CHO cell lines:

    RS-1102InstiGRO­™ CHO - 2 mL (Free sample)
    RS-1105InstiGRO® CHO - 5 mL
    RS-1125InstiGRO® CHO - 25 mL
    RS-1202InstiGRO® CHO PLUS - 2 mL (Free sample)
    RS-1205InstiGRO® CHO PLUS - 5 mL
    RS-1225InstiGRO­® CHO PLUS - 25 mL
    RS-2125InstiSHAKE™ CHO - 20 mL A + 5 mL B

    Support the development of biologics with HEK293 cell lines:

    RS-1302InstiGRO™ HEK - 2 mL (Free sample)
    RS-1305InstiGRO® HEK - 5 mL
    RS-1325InstiGRO® HEK - 25 mL
    RS-2325InstiSHAKE™ HEK - 25 mL
    RS-3305InstiTHAW™ HEK - 5 mL

    Services

    Offering a wide range of services including Advanced Care service agreements, Installation & Training packages and Validation reports, we keep customers productive and their results accurate, with top-level support at and beyond their purchase.

    Learn more

    The Insti products are the perfect solution for:

    • Supporting suspension CHO and HEK cell lines during cryopreservation, revival, single cell cloning, and expansion.
    • Supporting an animal component free workflow, whilst ensuring cells receive all the nutrition they need.
    Solentim VIPS® PRO
    High efficiency, single cell seeding and imaging platform

    High efficiency, single cell seeding with image-based proof of clonality for GMP-compatible workflows

    Learn more
    Solentim Cell Metric® X
    Clonal imager with automated assurance

    Simplify clonal assurance with Artificial Intelligence

    Learn more
    Solentim iPSC Matrices

    High Viability iPSC Single Cell Cloning and Outgrowth

    Learn more
    Application Note

    Addressing Cell Line Development Bottlenecks Progressing From Conditioned Medium To InstiGRO Reagents

    Download Now
    Webinar

    Healthy Cells, Healthy Results: How to Improve Cell Line Development

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    Application Note

    Single Cell Seeding Conditions For Improved Colony Outgrowth

    Download Now
    Certificates of Analysis

    Access Insti reagent Certificates of Analysis (COAs)

    Access COAs
    Instructions For Use

    Access Insti reagent Instructions For Use (IFUs)

    Access IFUs
    Application Note

    Improved Outgrowth For HEK 293 Cell Line Development

    Download Now

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    Austria: +49 695 0606 800
    Belgium: +31 6 899 25902
    France: +33 456 681 861
    Germany: +49 695 0606 800
    The Netherlands: +31 6 899 25902
    United Kingdom: +44 740 890 1366

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