Once opened, there is no need to rush to use the reagent. The supplement will still be valid for the full shelf life specified on the certificate of analysis.

No, the Insti reagents are not light sensitive.

The Insti HEK reagents (InstiGRO HEK, InstiSHAKE HEK, and InstiTHAW HEK) are all chemically defined. The Insti CHO reagnets (InstiGRO CHO, InstiGRO CHO PLUS, InstiSHAKE CHO, and InstiTHAW CHO) are not however.

No, filtering the reagents will not affect the performance of the product. If you wish to filter the reagents then this is not a problem, however it is not necessary as the product is supplied sterile.

All Insti reagents are manufactured at our site in the Southwest of the UK.

No, manufacturing grade InstiGRO, InstiSHAKE, and InstiTHAW are not commercially available at present. To help us understand your needs further, please contact your local representative from Advanced Instruments, or email us at [email protected].

All of the Insti products have been designed and validated for use with suspension  CHO and HEK cell lines. However, this is not to say that your cell line will not benefit from the support of these reagents. If you are interested in evaluating the benefits of the reagents with cell lines other than CHO or HEK, please email us at [email protected].

While the Insti CHO and HEK reagents have been designed and validated for suspension CHO and HEK cell lines, we have seen great results both internally and amongst our academic customer basis with adherent cell lines. For more information, please email us at [email protected].

The short answer is no – Every new batch of Insti reagent is subject to quality control checks to ensure that there is minimal lot to lot variability. Each new batch of reagent undergoes a functional test to ensure that its formulation and performance is consistent with the previous batch.

InstiGRO CHO, InstiGRO CHO PLUS, and InstiGRO HEK are all available in both 5 mL and 25 mL quantities. InstiSHAKE CHO and HEK are only available in 25 mL quantities, while InstiTHAW CHO and HEK are only available in 5 mL quantities.

No, simply add InstiGRO to your standard outgrowth medium, including any other additives or selective agents which are usually use.

No, you can simply expand clones using your standard growth medium – there is no need to ween the cells from the InstiGRO reagent. InstiGRO is however designed to support cells throughout the static culture phase of the workflow. It therefore may be beneficial to continue using InstiGRO throughout your static culture work.

Yes, as detailed on the Instructions for Use (IFU) document, cells should be cultured with a 1X concentration of instiGRO for 4-24 hours prior to single cell seeding.

No, simply add InstiSHAKE to your standard medium, including any other additives or selective agents which are usually used.

While InstiGRO CHO and CHO PLUS are both designed to support clonal outgrowth, the formulations are slightly different to cater for different cell lines and growth mediums. It is often difficult to predict which formulation will work best. We therefore recommend that all new customers evaluate clonal outgrowth with both formulations before deciding how to proceed.

No, InstiTHAW does not replace DMSO. InstiTHAW should be considered as an additional supplement to your standard cryoprotective solution.

Please email [email protected] if you have any further questions.