Cell counting and viability assessments are key parameters for monitoring cell health. The trypan blue assay is the earliest and most widely used method for quantifying live and dead cells. The Viability assay on the ICON instrument uses STUDIUS’ artificial Intelligence-based assessment to accurately identify cells when stained with trypan blue. Viability results can be combined with results of the low volume Titer assay, also available on ICON and analysed by the STUDIUS software to produce a measure of protein productivity. The Viability assay requires only 20μL of sample enabling stratification and selection earlier in the process, without using most of your cell culture. This is ideal for use with static plates or suspension culture deep well shaking plates, for both CHO and HEK cell lines. STUDIUS can import clonality results from the Cell Metric and combine them with ICON viability and titer data for fully automated clone selection, as well as track clones from seeding to final selection, providing a consistent audit trail to satisfy regulators. We will outline the importance of accuracy and efficiency when cell counting and outline how the Viability assay on the ICON can enhance your current processes.
Cell Metric is a high contrast imager designed for single cell imaging, identification and clonal outgrowth characterization.
Cell Metric captures and records crucial evidence, whole well image of the single cell at day 0 and daily imaging thereafter, all wrapped up in a clonality report defining best practice for Master Cell Bank production.
Available as a stand-alone single plate system, or with automated plate handling within a temperature-controlled environment from Cell Metric CLD or downstream third-party robotic integration.
Designed to meet the high sample throughput, regulatory compliance (including 21 CFR part 11), and data management needs of busy bioprocessing and biomanufacturing labs, the OsmoTECH PRO Multi-Sample Micro-Osmometer combines ease-of-use with accurate and precise osmolality measurements for reliable and reproducible results.
Optimize and ensure the quality and consistency of buffers, media, product, excipients, and process parameters to maximize biologic yield, quality, and purity.