OsmoTECH® HT Automated Micro-Osmometer

OsmoTECH^® HT Automated Micro-Osmometer

Optimize your high-throughput osmolality testing workflow

Automation-friendly 96-well plate-based format for high-throughput testing
Preserve sample with small testing volume
Powerful data integrity and management features for easy data tracking and compliance

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Equipment was easy to set up and easy to use. The [OsmoTECH HT] runs reliably and is great to have for running many samples.

Monica G / Biotech

Automation-friendly 96-well plate-based format
Small sample volume requirement
Reliable and consistent performance
Robust data integrity
Flexible and secure data management
Easy to use

Built for your process development and drug formulation needs

High-throughput bioprocess development involves many complex workflows. Between the need to perform osmolality testing on large numbers of samples and monitor multiple additional parameters, optimizing your lab’s workflows is critical. Automation-friendly features support your high-throughput environment, streamlining efficiency and reducing hands-on operator time.

Upstream Manufacturing Applications

Clone Screening

Speed to market, boost cell longevity, increase yield and reduce production costs — Know more about your clones sooner and select the best clones earlier in the process.

Cell Line Development

Boost cell longevity and maximize cell productivity — Optimize osmolality when developing new feeds and additives in cell culture to increase cell longevity.

Formulation Development

Accelerate formulation development and stability testing — Integrate into automated workflows providing excipient concentrations and confirm the soluble content of formulations.

Product Features

Plate-loading format allows samples to be robotically pipetted
96-well plate
Load up to two plates for 192 tests

Streamline efficiency
Reduce hands-on operator time
Seamless integration with automation workflows (Ambr®, Cedex®, and liquid handling automation systems)

Perform up to 1,000 tests before a consumables change is required
Uninterrupted sample testing overnight and weekends

Only 40 µL sample volume needed for early process osmolality testing
20 µL for STAT testing

Touchscreen interface
Step-by-step guides for tests
Easy access to test and consumable status

Seal plate with custom cover to prevent evaporation and ensure repeatable and reliable results

Save data to a network database, USB device or web server
Automate the process with bi-directional communication to eliminate transcription errors and save time
Easily integrate results into a data management system and batch records with OPC-UA

Automated sampling and fluid cleaning
Eliminate sample carryover and cross-contamination

Develop and store templates
Reduce set-up time

21 CFR part 11 compliant
Ease of implementation into a GMP environment

Purchase instruments with peace of mind
Knowledgeable, experienced, and responsive support team

Sample type	Aqueous-based solution
Sample volume per test	40 μL
Test time	140 seconds
Sample Capacity	Multi plate
Resolution	1 mOsm/kg H₂O
Range¹	0-2000 mOsm/kg H₂O
Accuracy² (AI standards and reference solutions)	Mean value ±3 mOsm/kg H₂O from nominal value between 0 and 400 mOsm/kg H₂O (1 SD) Mean value ±1% from nominal value greater than 400 to 2000 mOsm/ kg H₂O (1 SD)
Within-run Repeatability² (AI Standards and Reference Solutions)	Standard deviation ≤3 mOsm/kg H₂O between 0 and 400 mOsm/kg H₂O Coefficient of variation ≤1% greater than 400 to 2000 mOsm/kg H₂O
Operating conditions	Temperature: 18 °C to 35 °C (64 °F to 95 °F) Humidity: 5 to 80% relative humidity (non-condensing)
Supported Languages	English, Simplified Chinese, French, German, Italian, Japanese, Korean, Portuguese, Russian, Spanish, Turkish
Storage temperature	-20 °C to +45 °C (-4 °F to +113 °F)
Electrical voltage	100 to 240 VAC (50/60 Hz)
Power consumption	60 Watts
Dimensions (D x W x H)	48.9 cm D x 62.87 cm W x 68.58 cm H (19.25” D X 24.75” W X 27 H”)
Net weight	59.7 kg (131.6 lbs.)
Shipping weight	111.9 kg (246.6 lbs.)
Warranty	One-year limited warranty on workmanship and parts

Advanced Instruments completes testing from 0 mOsm/kg sample to 2000 mOsm/kg sample during in-house linearity testing.
Performance at reference conditions: 20 °C to 25 °C (68 °F to 77 °F); 30 to 60% relative humidity.

Osmometer Calibration Standards and Reference Solutions

SK-TECHHT	OsmoTECH HT Convenience Kit ( Includes 3MA635 , 635910, 635934 , 635920, 3MA029, 3LA028, 202850)
3MA635	OsmoTECH HT Calibration and Verification Standard Set

Supplies

635920	OsmoTECH HT 96 Well Plate Kit
635934	OsmoTECH HT System Fluid
635910	OsmoTECH HT Cleaning Reel
HT1000	OsmoTECH HT Micro-Sample Tubes, Pkg 1000
240821	Sampler Tips, 50/pkg

Accessories

135022	Epson Dot Matrix Printer (sold-separately)
AN2TP5	Dot Matrix Printer Paper
700056	Barcode scanner

Clinitrol™ 290 Reference Solution

10 x 2 mL Ampules

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Osmolality Linearity Set

0, 500, 900, 1500, 2000 mOsm/kg H₂O 5x 2x 2 mL Ampules

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Osmometer Calibration Standards

Available from 0-4000 mOsm/kg H₂O 10 x 2 mL Ampules

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Protinol™ Protein-Based Controls

Protinol 3-Level Osmometer Control 3x3x3 mL

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Biotech Services

Get the best performance from your osmometer from the people who designed and built it. We provide a range of services and service plans to meet the different needs of our customers. Our certified Field Service Engineers provide on-site professional support for the installation, training, validation, and maintenance of your osmometers.

Learn more

Protein Therapies

When it comes to process development and manufacturing of protein therapeutics, whether you’re making an antibody-based or other protein therapeutic, what you don’t know can slow you down.

Are all of your buffers and media formulated correctly?
Does your solution have unexpected components?
Are all of the steps involving solutions–i.e. cell growth, dialysis, chromatography–proceeding consistently and as expected?

With our innovative osmometers, you can gain valuable data for managing process and product quality through a simple 90-second test. Osmolality, the measurement of total solute concentration, is a reliable control specification and in-process parameter that can deliver information across the protein therapeutic process development and manufacturing workflow.

See all of our Osmometers

Application Notes

References

Amaral AI et al. Improving retroviral vectors production: Role of carbon sources in lipid biosynthesis. J Biotechnol. 2008 Nov 25;138(3-4):57-66.
Burger C et al. Systemic Mannitol-Induced Hyperosmolality Amplifies rAAV2-Mediated Striatal Transduction to a Greater Extent Than Local Co-infusion. Mol Ther. 2005 Feb;11(2):327-31.
Ferreira TB et al. Two different serum-free media and osmolality effect upon human 293 cell growth and adenovirus production. Biotechnol Lett. 2005 Nov;27(22):1809-13.
Rodrigues AF et al. Production of Retroviral and Lentiviral Gene Therapy Vectors: Challenges in the Manufacturing of Lipid Enveloped Virus. In Viral Gene Therapy, ed. Ke Xu. London:IntechOpen Limited. 2011.
Cordova A et al. Osmotic Shock and the Strength of Viral Capsids. Biophys J. 2003 Jul;85(1):70-4.
Coroadinha AS. Effect of Osmotic Pressure on the Production of Retroviral Vectors: Enhancement in Vector Stability. Biotechnol Bioeng. 2006 Jun 5;94(2):322-9.

Cell Therapies

When the therapeutic you’re developing is cell-based, constant monitoring to optimize growth and ensure consistent culture conditions throughout multiple stages is critical for success.

Are your stem cells at highest viability and product quality levels throughout culture and passaging?
Are you ensuring a high rate of cell recovery post-thaw?
Are the methods and processes used for transduction optimized for your system?

Using osmolality can help your group answer these questions early in the process, ultimately helping you save time, money, and limited or costly reagents. Adding a simple 90-second osmolality measurement to the in-process parameters you measure is a quick and easy way to ensure optimal cell growth, during process development and manufacturing as well as during buffer and media preparation.

With our innovative osmometers, you can keep delicate cells at optimal growth and gain valuable data for managing process and product quality across the cell therapy process development and manufacturing workflow.

See all of our Bioprocessing Osmometers

Application Notes

References

Casula E et al. Osmotic behaviour of human mesenchymal stem cells: Implications for cryopreservation. PLoS One. 2017 Sep 8;12(9):e0184180.
Li Y and Ma T. Bioprocessing of Cryopreservation for Large-Scale Banking of Human Pluripotent Stem Cells. Biores Open Access. 2012 Oct;1(5):205-14.
Petrenko Y et al. Clinically Relevant Solution for the Hypothermic Storage and Transportation of Human Multipotent Mesenchymal Stromal Cells. Stem Cells Int. 2019 Jan 20;2019:5909524.
Rafiq QA et al. The role of biopreservation in cell and gene therapy bioprocessing. Cell & Gene Therapy Insights. 2017. ISSN: 2059-7800. 335-344.
Bohnenkamp H et al. Bioprocess development for the cultivation of human T-lymphocytes in a clinical scale. Cytotechnology. 2002 Jan;38(1-3):135-45.
Nie Y et al. Scalable Passaging of Adherent Human Pluripotent Stem Cells. PLoS One. 2014 Jan 30;9(1):e88012.
Potočar U et al. Adipose-Derived Stem Cells Respond to Increased Osmolarities. PLoS One. 2016 Oct 5;11(10):e0163870.

Gene Therapies

Consistency is critical when producing genetic therapies, including viral vector delivery and gene editing. Small deviations in the composition of cell growth media can have unwanted effects on cell density, cell composition, and virus yield.

Are you looking for ways to increase vector stability?
Is your gene therapy group looking for higher transfection efficiency and higher viral vector production?

Using osmolality can help your group work towards those goals, as well as answer:

- how to optimize the physiological range for transfection complexes
- identifying media needs
- determining effects of osmotic pressure on the stability of a complex

With our innovative osmometers, you can gain valuable data for managing process and product quality through a simple 90-second test. When done right, osmolality, the measurement of total solute concentration, is a reliable control specification and in-process parameter that can deliver information across the gene therapy process development and manufacturing workflow.

See all of our Osmometers

Application Notes

References

Amaral AI et al. Improving retroviral vectors production: Role of carbon sources in lipid biosynthesis. J Biotechnol. 2008 Nov 25;138(3-4):57-66.
Burger C et al. Systemic Mannitol-Induced Hyperosmolality Amplifies rAAV2-Mediated Striatal Transduction to a Greater Extent Than Local Co-infusion. Mol Ther. 2005 Feb;11(2):327-31.
Ferreira TB et al. Two different serum-free media and osmolality effect upon human 293 cell growth and adenovirus production. Biotechnol Lett. 2005 Nov;27(22):1809-13.
Rodrigues AF et al. Production of Retroviral and Lentiviral Gene Therapy Vectors: Challenges in the Manufacturing of Lipid Enveloped Virus. In Viral Gene Therapy, ed. Ke Xu. London:IntechOpen Limited. 2011.
Cordova A et al. Osmotic Shock and the Strength of Viral Capsids. Biophys J. 2003 Jul;85(1):70-4.
Coroadinha AS. Effect of Osmotic Pressure on the Production of Retroviral Vectors: Enhancement in Vector Stability. Biotechnol Bioeng. 2006 Jun 5;94(2):322-9.

Personal Care

Osmolality testing has unlimited applications in areas of personal care and hygiene. Products that come into contact with or penetrate the skin must have an osmolality within a physiological range to avoid damaging cells and tissues. In some instances, slightly hypo- or hyper-osmotic solutions are generated to promote wetting or drying effects. The manufacturing of skincare products, personal lubricants and optical products

One key to a youthful look is healthy and moisturized skin. Proper hydration of skin depends on a balance of water flow into and out of skin cells and, thus, depends on osmolality. Osmolality testing of skincare products provides assurance that the product will have moisturizing effects and serves as a control during the production process.

The osmolality of personal lubricant formulations impacts their effect and safety. The World Health Organization (WHO) stated that the osmolality of these solutions should not exceed 1200 mOsm/kg, with this limit decreasing to a more iso-osmotic level in the future.

Similar value for osmolality testing can be clearly seen in optical applications. Eye drops and other optical products are designed to lubricate or protect the eye. The goal of these solutions (e.g. adding moisture, removing moisture, etc.) dictates an ideal osmolality range.

See all of our Osmometers

References

Aragona P et al. Physiochemical Properties of Hyaluronic Acid-Based Lubricant Eye Drops. Trans Vis Sci Tech. 2019;8(6):2.
Corrales R et al. Effects of Osmoprotectants on Hyperosmolar Stress in Cultured Human Corneal Epithelial Cells. Cornea 2008;27:574-579.
WHO/UNFPA/FHI, 2011-. Advisory Committee Notes to the Female Condom Technical Review Committee Meeting. [Geneva, Switzerland].

Vaccines

Osmolality testing is required to ensure that the final vaccine product is safe for administration. The osmolality must be within a physiological range to be introduced in human tissues. There is also evidence that injectable formulations with high osmolality may cause pain at the site of injection.

Vaccines are derived from a variety of sources, both living and synthetic, meaning a variety of bioproduction workflows are required. These workflows include some common use cases for osmolality testing, including the following:

Upstream cell culture and/or cell expansion requires maintaining an environment at the appropriate osmolality for the specific cell type. For those modalities that are cell-based, regular osmolality testing will ensure that the media and environment are optimized.
Buffers used during any downstream processing should be tested for osmolality to verify the concentrations of the solutions that are meant to protect the drug substance throughout the chromatography and/or filtration operations.
Final product testing must include osmolality to verify identify and safety of any injectable.

Differentiation in use cases for osmolality testing are outlined below.

Differentiation in use cases for osmolality testing are outlined below:

Monoclonal Antibody Vaccines

Osmolality testing should be performed on hybridoma cell media to ensure optimal antibody production rates. The rate of antibody production in hybridoma cells depends on the osmolality of the medium in which the cells are grown. The osmotic agent used to affect the osmolality levels also impacts Ab production.

Osmolality testing should be performed on hybridoma cell media to ensure optimal antibody production rates.
The rate of antibody production in hybridoma cells depends on the osmolality of the medium in which the cells are grown.
The osmotic agent used to affect the osmolality levels also impacts Ab production.

Viral Vaccines

Osmolality testing should be performed on media solutions to optimize cell size and viral vector stability. Osmotic pressure affects the size of the morphology of insect cells and viruses. The osmolality of the media affects the composition of the lipid bilayer membrane of enveloped viruses. This directly impacts the vector stability.

Osmolality testing should be performed on media solutions to optimize cell size and viral vector stability.
Osmotic pressure affects the size of the morphology of insect cells and viruses.
The osmolality of the media affects the composition of the lipid bilayer membrane of enveloped viruses. This directly impacts the vector stability.

mRNA and DNA Vaccines

Delivery systems and mechanisms should be tested for osmolality. mRNA can be complexed with different agents (e.g. PEI) for delivery within the body. Osmolality impacts the stability of the complex of viral models. Osmolality impacts the ability to penetrate the target cell membrane.

Delivery systems and mechanisms should be tested for osmolality.
mRNA can be complexed with different agents (e.g. PEI) for delivery within the body. Osmolality impacts the stability of the complex of viral models.
Osmolality impacts the ability to penetrate the target cell membrane.

See all of Our Osmometers

References

Hasan et al. Osmolytes in vaccine production, flocculation and storage: a critical review. I. 2019; 15(2): 514–525.
Coroadinha, AS et al. Effect of osmotic pressure on the production of retroviral vectors: Enhancement in vector stability. Biotechnol Bioeng 2006; 94(2):322-9.
Tanja A. Grein, Tobias Weidner and Peter Czermak. Concepts for the Production of Viruses and Viral Vectors in Cell Cultures. New Insights into Cell Culture Technology, pp.173-191.

Pardi, N., Hogan, M., Porter, F. et al. mRNA vaccines — a new era in vaccinology. Nat Rev Drug Discov 17, 261–279 (2018).
Choi H-J, Song J-M, Bondy BJ, Compans, RW, Kang S-M, Prausnitz MR (2015). Effect of Osmotic Pressure on the Stability of Whole Inactivated Influenza Vaccine for Coating on Microneedles. PLoS ONE 10(7): e0134431.
Rosinski, M. Osmolarity effects on observed insect cell size after baculovirus infection are avoided using growth medium for sample dilution. Biotechnol Prog. 2000 Sep-Oct;16(5):782-5.
Sonia Gutiérrez-Granados, Laura Cervera, Amine A. Kamen & Francesc Gòdia (2018): Advancements in mammalian cell transient gene expression (TGE) technology for accelerated production of biologics, Critical Reviews in Biotechnology.

Oligonucleotides

This novel field involves the use of antisense oligonucleotides (ASOs), aptamers, siRNAs, miRNAs and synthetic mRNAs to control the expression of genes that have been associated with diseases. Multiple Advanced Instruments customers within this field use osmometers to verify the concentration of the solutions carrying their precious products. Ultimately, the quality of the drug is dependent on the process controls implemented throughout the workflow. Below are some of the current uses for osmolality testing in this field:

Buffer QC

Osmolality specifications for buffers to preserve and protect the critical quality attributes of the product as it is being developed.

Final Product

Compendial release test needed to confirm the identity and concentration of the oligo product for safe use.

Because this is an emerging field, it comes with a number of development and application challenges:

Delivery of RNAs across the lipid bilayer and into cells
Immunogenicity once in the body
High rates of degradation of oligos by plasma and tissues

Drug delivery into the target cells requires the proper osmotic pressure, similar to the parameters around viral transfection in vector development. The optimal osmolality of the delivery complex (e.g. mRNA:PEI) should be determined and monitored during this process. This specification will provide repeatable successful delivery of the drug.

See all of our Osmometers

References

Pardi, N., Hogan, M., Porter, F. et al. mRNA vaccines — a new era in vaccinology. Nat Rev Drug Discov 17, 261–279 (2018)
Poveda C, Biter AB, Bottazzi ME, Strych U. Establishing Preferred Product Characterization for the Evaluation of RNA Vaccine Antigens. Vaccines (Basel). 2019 Sep 27;7(4). pii: E131.

All models support Pharmacopeia testing guidelines	New! OsmoTECH^® HT Automated Micro-Osmometer	OsmoTECH^® PRO Multi-Sample Micro-Osmometer	OsmoTECH^® XT Single-Sample Micro-Osmometer	OsmoTECH^® Single-Sample Micro-Osmometer	Model 3250 Single-Sample Osmometer Being discontinued Dec. 31, 2020
Best for labs with daily sample processing needs of:	>20/day	>10/day	1-10/day	1-10/day	1-10/day
Sample volume	40 µL	30 µL	20 µL	20 µL	250 µL
Sample introduction	96 well plate	Sample cup	One-step direct	One-step direct	Sample cup
Operating range (mOsm/kg H₂O)	0 - 2,000 mOsm/kg H₂O	0 - 2,000 mOsm/kg H₂O	0 – 4,000 mOsm/kg H₂O	0 - 2,000 mOsm/kg H₂O	0 - 4,000
Test time (seconds)	140	90	Less than 150 in low range, Less than 190 in high range	90	120
Multi-sample capacity
Automated cleaning between samples
Complex Matrices (High Concentrations, Sugars, & Proteins)
Integrated barcode scanner
Onboard Video Instructions
Multi-language touchscreen display
Supports 21 CFR Part 11 & EU Annex 11 compliance
Electronic Signature (Review & Approve)
Supports Pharmacopeia testing guidelines compliance
Username & Password Compatibility (With Active Directory)
Dot-matrix Printer
Network Connection
Network share
Automatic backup
Control device remotely via OPC-UA
USB
Webserver for remote data viewing
Data Storage Capacity	Unlimited	Unlimited	Unlimited	Unlimited
Levels of users access	3 Levels	3 Levels	3 Levels	3 Levels
Data Format	.csv & pdf	.csv & pdf	.csv & pdf	.csv & pdf
Part #	OSMOTECHHT	OsmoTECHPRO	OsmoTECHXT	OsmoTECH	3250
Download Chart as a PDF Sold separately	Learn More Request Pricing	Learn More Request Pricing	Learn More Request Pricing	Learn More Request Pricing	Learn More Request Pricing

Application Note

Delivering Reliable Performance Of Osmolality Testing With High Throughput Automation

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Poster

Product In Action

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Application Note

Osmolality as a concentration measurement method for key buffers in bioprocessing

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Webinar

Maintaining Data Integrity And Compliance Of Your Osmometer

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Knowledge Center

What Is Osmolality?

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Webinar

Introduction Of New OsmoTECH® HT Automated Micro-Osmometer

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What our customers say

Equipment was easy to set up and easy to use. The [OsmoTECH HT] runs reliably and is great to have for running many samples.

Monica G

Biotech

Request pricing

For a quotation, to arrange a demonstration, or for more information please contact us by phone or complete the form.

Phone: +1-781-320-9000
Toll free: 800-225-4034 (US & Canada)

Austria: +49 695 0606 800
Belgium: +31 6 899 25902
France: +33 456 681 861
Germany: +49 695 0606 800
The Netherlands: +31 6 899 25902
United Kingdom: +44 740 890 1366

China: +86 21 3412 6167

Download Brochure

Please fill out this brief form to download this content. You can also get in touch with our sales team for product questions, arranging a product demonstration or obtaining a quotation at the phone numbers below:

Phone: +1-781-320-9000
Toll free: 800-225-4034 (US & Canada)

Austria: +49 695 0606 800
Belgium: +31 6 899 25902
France: +33 456 681 861
Germany: +49 695 0606 800
The Netherlands: +31 6 899 25902
United Kingdom: +44 740 890 1366

China: +86 21 3412 6167

Request a Virtual Demo

For a quotation, to arrange a demonstration, or for more information please contact us by phone or complete the form.

Phone: +1-781-320-9000
Toll free: 800-225-4034 (US & Canada)

Austria: +49 695 0606 800
Belgium: +31 6 899 25902
France: +33 456 681 861
Germany: +49 695 0606 800
The Netherlands: +31 6 899 25902
United Kingdom: +44 740 890 1366

China: +86 21 3412 6167

Compliance & Conformity

Compliance & Conformity