|Solution Range (μL)||(Uncertainty) Inaccuracy(%)||(Imprecision) CV (%)|
|PCRMix B||49.99 to 10.00||1.54 to 1.90||0.15 to 0.20|
|PCRMix C||9.999 to 2.000||1.46 to 1.63||0.15 to 0.20|
PCRMix QualAssure are solutions for MVS that have been manufactured with a proprietary formulation that includes some of the main constituents that comprise commercial master mix. PCRMix is built to mimic the rheological properties of commercial master mix. It is an “off the shelf” solution that allows you to verify the volume of master mix that is dispensed by your automated liquid handler. Regular aqueous solutions are too different to be used for optimization of master mix liquid classes. While it is possible to make a more viscous MVS solution using the Alternative Solution Helper, that can be time-consuming to do, and you don’t really know if your viscous Alternative Solution mimics the behavior of master mix. PCRMix can save money by decreasing the amount of time you spend optimizing your liquid handler and preventing costly pipetting mistakes.
Figure 2. This data demonstrates a comparison of PCRMix Sample Solution to the pipetted volumes of leading commercial master mix at ~4 °C using an ALH optimized with PCRMix Sample Solution.
Figure 1. This data demonstrates the difference in dispensed volume with Aqueous QualASsure and PCRMix QualAssure at ~4 °C using an ALH optimized with PCRMix QualAssure solution to dispense 9.9 µL.
The volume of PCR master mix can have a direct effect on qPCR results. qPCR reaction accuracy is dependent on the master mix performance and the volume added. Under-delivery of master mix can lead to unexpected Cq values. In this example, if the scientist had accidentally underdelivered the master mix by 40% (they should have delivered 10 µL but actually delivered 6 µL) the results would have yielded a Cq value significantly higher than if the correct volume of master mix had been dispensed. Falsely high Cq values translates to improper quantification of gene of interest in the sample by producing a false low concentration. This further translates to the possibility of mis-diagnosing a patient. All because an incorrect volume of master mix had been dispensed.
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