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PCRMix™ QualAssure®

Optimize the dispensing steps of your assays without wasting expensive reagents.
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Optimize the dispensing steps of your PCR, qPCR, RT-PCR and NGS assays without wasting expensive reagents.

PCRMix QualAssure solutions for the MVS have been manufactured with a proprietary formulation that includes some of the main constituents of commercial master mix. It is an “off the shelf” solution that simplifies performance optimization by allowing you to verify the volume of master mix dispensed by your automated liquid handler. PCRMix QualAssure can ensure accuracy and precision of the volumes of master mix being dispensed, thereby preventing costly pipetting mistakes, and decreasing variability in your PCR assay.​

Highlighted features
  • Convenient
  • Easy-to-Use
  • Designed specifically for Artel MVS

PCRMix QualAssure are solutions for MVS that have been manufactured with a proprietary formulation that includes some of the main constituents that comprise commercial master mix. PCRMix is built to mimic the rheological properties of commercial master mix. It is an “off the shelf” solution that allows you to verify the volume of master mix that is dispensed by your automated liquid handler. Regular aqueous solutions are too different to be used for optimization of master mix liquid classes. While it is possible to make a more viscous MVS solution using the Alternative Solution Helper, that can be time-consuming to do, and you don’t really know if your viscous Alternative Solution mimics the behavior of master mix. PCRMix can save money by decreasing the amount of time you spend optimizing your liquid handler and preventing costly pipetting mistakes.


Figure 2. This data demonstrates a comparison of PCRMix Sample Solution to the pipetted volumes of leading commercial master mix at ~4 °C using an ALH optimized with PCRMix Sample Solution.


Figure 1. This data demonstrates the difference in dispensed volume with Aqueous QualASsure and PCRMix QualAssure at ~4 °C using an ALH optimized with PCRMix QualAssure solution to dispense 9.9 µL.

Under-Delivery of qPCR Master Mix Alters Assay Results

The volume of PCR master mix can have a direct effect on qPCR results. qPCR reaction accuracy is dependent on the master mix performance and the volume added. Under-delivery of master mix can lead to unexpected Cq values. In this example, if the scientist had accidentally underdelivered the master mix by 40% (they should have delivered 10 µL but actually delivered 6 µL) the results would have yielded a Cq value significantly higher than if the correct volume of master mix had been dispensed. Falsely high Cq values translates to improper quantification of gene of interest in the sample by producing a false low concentration. This further translates to the possibility of mis-diagnosing a patient. All because an incorrect volume of master mix had been dispensed.

PCRMix QualAssure solutions will help you:

  • Ensure master mix delivery volume is accurate and precise
  • Decrease variability of PCR assay results and even false results

96 Well MVS Verification Plate

Solution Range (μL) (Uncertainty) Inaccuracy(%) (Imprecision) CV (%)
PCRMix B 49.99 to 10.00 1.54 to 1.90 0.15 to 0.20
PCRMix C 9.999 to 2.000 1.46 to 1.63 0.15 to 0.20
MVS® Multichannel Verification System

Simple volume verification with results in less than 10 minutes.

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Aqueous™ QualAssure®

Assists in liquid class optimization for water-based solutions.

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PlasProxy™ QualAssure®

Mimics various animal and human plasma in assay verification.

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DMSO QualAssure®

Validates liquid transfers whenever dimethyl sulfoxide solvent is used.

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SerumSub™ QualAssure®

Acts as a substitute for serum in your most critical assays.

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Services

Offering a wide range of services including Advanced Care service agreements, Installation & Training packages and Validation reports, we keep customers productive and their results accurate, with top-level support at and beyond their purchase.

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White Paper

Using PCRMix™ QualAssure™ for Assessing Liquid Transfer Performance with the MVS®

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Webinar

The Key To Assay Reproducibility: Viscous Liquid Optimization

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For a quotation, to arrange a demonstration, or for more information please contact us by phone or complete the form.

Austria: +49 695 0606 800
Belgium: +31 6 899 25902
France: +33 456 681 861
Germany: +49 695 0606 800
The Netherlands: +31 6 899 25902
United Kingdom: +44 740 890 1366